[Histonet] Re: Histonet Digest, Vol 80, Issue 11

Brian Johnson brianj18 <@t> u.washington.edu
Fri Jul 9 12:38:08 CDT 2010


Hi Karol

I just wanted to follow up with you regarding the Fluorescent secondary antibodies and if you needed to order them or not.  Can I come by your lab to chat for a minute?  Where are you located?

Brian

Brian Johnson
Research Scientist
U.W. at South Lake Union, N310
HIC/Comparative Pathology Program
815 Mercer St
Seattle, WA 98109
Work Phone: 206-685-6517
Work E-mail: brianj18 <@t> uw.edu

On Jul 9, 2010, at 10:01 AM, histonet-request <@t> lists.utsouthwestern.edu wrote:

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> Today's Topics:
> 
>   1. RE: Peloris Users (Tapper, Sheila J.)
>   2. AP Computer Systems (jeff)
>   3. Histo bath (jeff)
>   4. Helf- strange IF result (sharon in)
>   5. Laura Miller is Out of  the Office.
>      (Laura.Miller <@t> leica-microsystems.com)
>   6. PGP 9.5 Frozen Tissue (DianaRip1 <@t> aol.com)
>   7. VIP 2000 (sgoebel <@t> xbiotech.com)
>   8. RE: SPAM-LOW:  RE: [Histonet] Reprocess (Patsy Ruegg)
>   9. Re: VIP 2000 (Drew Meyer)
>  10. Correct CPT? (Sara Baldwin/mhhcc.org)
>  11. Anybody running RCC on Bond (Dana Settembre)
>  12. RE: VIP 2000 (Bell, Lynne)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Thu, 8 Jul 2010 12:47:58 -0500
> From: "Tapper, Sheila J." <STapper <@t> smdc.org>
> Subject: RE: [Histonet] Peloris Users
> To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<A72CC3753325E4448AE0A7A3C46AD6DB056E130F <@t> BOREAL.ntcampus.smdc.org>
> Content-Type: text/plain;	charset="US-ASCII"
> 
> I you are going to choose to override the prompts, you had best revert
> back to a schedule of changing solutions on a rotating schedule.  
> You probably had something like that in place with your previous
> processor.  I would call Leica to have them send out someone to help get
> your processing optimized, and train your staff on how to set the
> parameters for your solutions.  They are very interested in keeping
> their customers happy.
> 
> Sheila 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Sharon.Davis-Devine
> Sent: Thursday, July 08, 2010 8:51 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Peloris Users
> 
> To all you Peloris users, here is my delimma.  When we follow the
> machines prompt to change the solutions because of purity and we then
> process small biopsies on the machine, it overprocesses the specimens.
> So the staff have been overrriding the prompt to change the solutions
> causing specimens to be processed in impure solutions causing a host of
> other problems. Have any of you out there with Peloris experience had
> this problem? Is it a machine problem that should be fixed or human
> error due to overriding the prompts?  I am new to this position and have
> very little experience with this issue so any help or suggestions will
> be greatly appreiciated. Thank you.
> 
> Sharon Davis-Devine, CT (ASCP)
> Cytology-Histology  Supervisor
> Carle Foundation Hospital
> Laboratory and Pathology Services
> 611 West Park Street
> Urbana, Illinois 61801
> 217-383-3572
> sharon.davis-devine <@t> carle.com
> 
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Thu, 8 Jul 2010 11:51:07 -0700
> From: "jeff" <jlhowery <@t> yrmc.org>
> Subject: [Histonet] AP Computer Systems
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000601cb1ece$86516a90$3394640a <@t> yrmc.org>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> We are currently looking at a overall computer system for our Hospital. My point of this question is has anyone worked with and their feelings about the following vendors for the AP application:
> Meditech
> Cerner
> Quadra Med
> 
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> 
> 
> ------------------------------
> 
> Message: 3
> Date: Thu, 8 Jul 2010 11:56:53 -0700
> From: "jeff" <jlhowery <@t> yrmc.org>
> Subject: [Histonet] Histo bath
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000601cb1ecf$545807f0$3394640a <@t> yrmc.org>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> I am looking for a condenser cooling fan motor for my Histo -Bath. Thank you for any Help .
> 
> Confidentiality Notice: This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
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> 
> ------------------------------
> 
> Message: 4
> Date: Thu, 8 Jul 2010 22:31:58 +0300
> From: sharon in <sharonin1 <@t> gmail.com>
> Subject: [Histonet] Helf- strange IF result
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<AANLkTiml3mtfOA2ptu5N1496GzkBqixoHG90MTAIk1o9 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Hi,
> 
> I am detecting Arc (Activity regulated cytoskeletal protein) protein in rat
> brain slices.
> I perfuse transcardially the animal with 0.1M PBS and than 4% PFA with 5%
> sucrose. Post fixation in 30% sucrose 1% PFA. In some cases when i take out
> the brain after the perfusion i make small cut or incision with the skull in
> the motor cortex, usually at the right side of the brain. I stain coronal
> 40um brain slices with primary antibody (from rabbit , santa cruz) and
> secondary anti rabbit Alexa488. I found very strong nuclear staining in the
> area around the cut in the motor cortex something like 100 um from each
> side. Do somebody has any idea what is the source of that staining?
> Generally the signal of Arc is in the nucleus and dendrites in other brain
> regions. I have very consistent results with Arc expression in the brain
> regions i am working on. The activation around the cutting area makes me
> worry a little.
> Thank you very much.
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Thu, 8 Jul 2010 16:48:53 -0500
> From: Laura.Miller <@t> leica-microsystems.com
> Subject: [Histonet] Laura Miller is Out of  the Office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OFE3CED835.2DB37FDC-ON8625775A.0077D4FB-8625775A.0077D4FB <@t> leica-microsystems.com>
> 	
> Content-Type: text/plain; charset=US-ASCII
> 
> 
> I will be out of the office starting  07/08/2010 and will not return until
> 07/09/2010.
> 
> I am out of the office for the rest of today.  I will reply tomorrow,
> Friday,  July 9th.
> 
> 
> ______________________________________________________________________
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> 
> 
> ------------------------------
> 
> Message: 6
> Date: Thu, 8 Jul 2010 21:09:41 EDT
> From: DianaRip1 <@t> aol.com
> Subject: [Histonet] PGP 9.5 Frozen Tissue
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4a71f.2740e446.3967d0d5 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
> 
> Is anyone out there doing the PGP 9.5 on fresh or frozen  tissue?
> 
> 
> ------------------------------
> 
> Message: 7
> Date: Fri, 09 Jul 2010 07:15:13 -0700
> From: sgoebel <@t> xbiotech.com
> Subject: [Histonet] VIP 2000
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<20100709071513.9e2d9aa830e8449a2412eb1e4f2f067e.77a782036c.wbe <@t> email04.secureserver.net>
> 	
> Content-Type: text/plain; charset="utf-8"
> 
> 
>   Hello all,
> 
>   I  recently  purchased a refurbished VIP.    3rd  time  I  have  run it, but wait...diagnose flas   error  code 73.  Of course the manual doesn't say what   to  fix it, and I can't seem to find anything online either.   this  is  an  older  machine  and lots of you have used it, was ju   wondering if anyone knew what the heck diagnose code 73 is?
> 
>   Th
>   Sarah Goebel, B.A., HT (ASCP)<   Histotechnician
>   XBiotech USA Inc.
>   8201 E   <   (512)386-51
> 
> ------------------------------
> 
> Message: 8
> Date: Fri, 9 Jul 2010 08:26:18 -0600
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: SPAM-LOW:  RE: [Histonet] Reprocess
> To: <histotech <@t> imagesbyhopper.com>, "'Tony Henwood'"
> 	<AnthonyH <@t> chw.edu.au>,	<sgoebel <@t> xbiotech.com>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6CEEF65C14414251AA4D2424461FE035 <@t> prueggihctechlt>
> Content-Type: text/plain;	charset="us-ascii"
> 
> I do something similar but I do melt the block down and dab out the melted
> paraffin with paper towel, put the melted tissue in a cassette and
> reprocess, it works a charm.  No need to remove paraffin.
> 
> Patsy
> 
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net 
> www.ihcrg.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histotech <@t> imagesbyhopper.com
> Sent: Thursday, July 08, 2010 7:02 AM
> To: 'Tony Henwood'; sgoebel <@t> xbiotech.com; histonet <@t> lists.utsouthwestern.edu
> Subject: SPAM-LOW: RE: [Histonet] Reprocess
> 
> What Tony suggests is similar to what we do for reprocessing.  If we have a
> block that we embedded and attempted to section, only to discover that it's
> not fixed well enough, we do the following:
> 
> 1) print a new processing cassette
> 2) using a "dull" knife (we have an old, dull steak knife), run the blade
> between the edge of the block and the cassette and "shave" the embedded
> tissue off the cassette.
> 3) take the wax/tissue, place in the new cassette and process.
> 
> No need to melt it down or run it back down from xylene to formalin.  The
> moist tissue is already exposed and seems to re-process just fine.
> 
> Your mileage may vary!
> 
> Michelle
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
> Sent: Wednesday, July 07, 2010 7:48 PM
> To: sgoebel <@t> xbiotech.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Reprocess
> 
> 
> You do not need to rehydrate the blocks back to water, 
> 
> While the tissue is still hot (ie the wax is still molten) blot the tissue
> dry, place it back in its cassette and place the cassette in 10% formalin
> for reprocessing. This procedure dramatically improves the results in at
> least 90% of cases. The excellent results are probably due to the protective
> nature of the wax present in the adequately processed portions of the block.
> This insulates the tissue from the harmful effects of ethanol on the
> adequately processed portions of the tissue preventing the tissue from
> becoming hard and brittle (Johnson 2003).
> 
> Johnson (2003) Histologic 36(1):21-22.
> 
> Regards
> 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager &
> Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead 
> Cnr Hawkesbury Road and Hainsworth Street, Westmead 
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> sgoebel <@t> xbiotech.com
> Sent: Thursday, 8 July 2010 1:37 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Reprocess
> 
> 
> 
>   Hello  all!!  Hope everyone had a happy 4th!!  Question =oday is...I
>   grossed  in  some  fat  that I need to routine process.  I ha=e done
>   this  in  the  past  with  extra fixation and no problem?  This tim=
>   however  it didn't fix all the way through and I have oily unfixed fat
>   in  =the  middle  of  my  block.  I fixed for 48 hours, but guess my
>   section  we=re  too big and needed more.  I want to fix for a little
>   longer  and rep=ocess the block.  I know I need to melt it down, but
>   then  what? =I  have done this before, it's just my brain is getting
>   older =)
> 
>   Thanks in advance!!
> 
>   Sarah Goebel, B.A., HT (=SCP)
> 
>   Histotechnician
> 
>   XBiotech USA Inc.
> 
> 
>   8201 East Riverside Dr. Bldg 4 Suite 100
> 
>   Austin, Texas  78744
> 
>   (512)386-5107 _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ****************************************************************************
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> If you are not the intended recipient, please delete it and notify the
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> Views expressed in this message and any attachments are those of the
> individual sender, and are not necessarily the views of The Children's
> Hospital at Westmead
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> 
> 
> 
> ------------------------------
> 
> Message: 9
> Date: Fri, 9 Jul 2010 11:35:06 -0400
> From: Drew Meyer <41dmb41 <@t> gmail.com>
> Subject: Re: [Histonet] VIP 2000
> To: sgoebel <@t> xbiotech.com
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<AANLkTikB7T_ScqqRQ_jfvJ_3L6C8kJRTo2Xt7WiBXR23 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> You can always call Sakura... they'll at least tell you what the codes
> means...
> 
> Drew
> 
> On Fri, Jul 9, 2010 at 10:15, <sgoebel <@t> xbiotech.com> wrote:
> 
>> 
>>  Hello all,
>> 
>>  I  recently  purchased a refurbished VIP.  ; This would have been the
>>  3rd  time  I  have  run it, but wait...diagnose flas hes and I get an
>>  error  code 73.  Of course the manual doesn't say what this is or how
>>  to  fix it, and I can't seem to find anything online either.   I know
>>  this  is  an  older  machine  and lots of you have used it, was ju st
>>  wondering if anyone knew what the heck diagnose code 73 is?
>> 
>>  Th anks
>> 
>>  Sarah Goebel, B.A., HT (ASCP)< /div>
>>  Histotechnician
>>  XBiotech USA Inc.
>>  8201 E ast Riverside Dr. Bldg 4 Suite 100
>>  < i>A ustin, Texas  78744
>>  (512)386-51 07
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> 
> ------------------------------
> 
> Message: 10
> Date: Fri, 9 Jul 2010 11:50:02 -0400
> From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
> Subject: [Histonet] Correct CPT?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OF4F3B140F.0CA25B7C-ON8525775B.0056FA65-8525775B.0056FA68 <@t> LocalDomain>
> 	
> Content-Type: text/plain;	charset=ISO-8859-1
> 
> Hey histoland
> When we do an FNA we sometimes have a cell block and we bill CPT 88160 and 88305
> These are sometimes kicked back to us and we put a 59 modifier on the 88305 then it goes thru.  Are we doing this correct ?  Any advice?
> 
> Thanks
> Pathology Supervisor
> Kathy Baldwin, SCT (ASCP)
> Memorial Hospital and Health Care Center
> sbaldwin <@t> mhhcc.org
> Ph 812-482-0210, 482-0216,  Fax 812-482-0232, 
> Pager 812-481-0897
> Confidential information, Authorized use only.
> 
> 
> 
> ------------------------------
> 
> Message: 11
> Date: Fri, 09 Jul 2010 12:14:08 -0400
> From: "Dana Settembre" <settembr <@t> umdnj.edu>
> Subject: [Histonet] Anybody running RCC on Bond
> To: "Alyssa Peterson" <alyssa <@t> alliedsearchpartners.com>,
> 	<histonet <@t> lists.utsouthwestern.edu>, "Dana Settembre"
> 	<settembr <@t> umdnj.edu>
> Cc: Catherine Susan Delia <Deliacs <@t> umdnj.edu>,	Neena Mirani
> 	<miraninm <@t> umdnj.edu>
> Message-ID: <sc37129d.052 <@t> smtpnpc.umdnj.edu>
> Content-Type: text/plain; charset=US-ASCII
> 
> Is anybody running RCC (Renal Cell Carcinoma) on Leica's Bond Max on
> human
> tissue?
> I am trying to bring it into our lab.
> I can't seem to get it working. 
> Normal tissue is staining but not tumor.
> I am using Lieca's concentrated antibody.
> Help.
> What is your Vendor?
> 
> Thank you,
> 
> Dana Settembre, HT ASCP
> Immunohistochemistry Lab
> UMDNJ - University Hospital
> Newark, NJ    USA
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> ------------------------------
> 
> Message: 12
> Date: Fri, 9 Jul 2010 12:25:25 -0400
> From: "Bell, Lynne" <Lynne.Bell <@t> cvmc.org>
> Subject: RE: [Histonet] VIP 2000
> To: "sgoebel <@t> xbiotech.com" <sgoebel <@t> xbiotech.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<F9E5562685AF9B498D9D8C1EC996D2440F52FE3CF1 <@t> cvmc-email.CVMC.ORG>
> Content-Type: text/plain; charset="us-ascii"
> 
> Error code 73:
> Symptom: Rotary valve does not stop at the required station after 2 times rotation
> Probable cause: 1. Photointerrupter defective
>                 2. Positioning disc broken
>                 3.  Photointerrupter for position detection disturbed by strong light                source
> Remedy: 1. Replace photointerrupter PC board
>          2. Replace positioning disc
>          3.  Eliminate light source
> 
> Just found this information in my older VIP E300 manual.
> 
> Lynne A. Bell, HT (ASCP)
> Technical Specialist, Histology
> Central Vermont Medical Center
> 130 Fisher Road
> Barre, VT  05641
> 802-371-4923
> 
> 
> 
> 
> ------------------------------
> 
> _______________________________________________
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> 
> End of Histonet Digest, Vol 80, Issue 11
> ****************************************



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