[Histonet] Gout Crytals

[Joyce Cline]

This works for us.
Process normally, we usually fix in 100% alcohol.
Cut slide
Formula 83 or Xylene 20 seconds
Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds
Eosin for 20 seconds
100% alcohol 20 seconds
100% alcohol 20 seconds
Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds
Form 83 or Xylene for 20 seconds
coverslip normally

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab [SohrabB1 <@t> ah.org]
Sent: Tuesday, July 06, 2010 4:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gout Crytals

Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.?
Thank you
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