[Histonet] Re: Turtox Internal Ear, cochlea slides

Tue Jul 6 07:21:51 CDT 2010

Hi Michelle,

I worked for a company called WARD'S Natural Science in Rochester, NY; they produce/provide science education materials.  I can't say I ever produced the internal ear slide myself, but I can recall others in the department working on something similar.  I would give them a call at 1-800-962-2660 and ask to speak with a tech or supervisor in the microscope slides department.  Even if they don't normally carry what you're looking for, I am sure they would be happy to try and provide you with what you want.

I hope you find what you're looking for!  Best of luck.

Adrienne

Adrienne Aperghis Kavanagh 
US PATH 
30 W. Century Road 
Suite 255 
Paramus NJ 07652 

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Subject: Histonet Digest, Vol 80, Issue 5

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Today's Topics:

1. RE: SPAM-LOW: [Histonet] Re: replacement Turtox Internal
Ear, cochlea slides (Patsy Ruegg)
2. RE: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks
(Patsy Ruegg)
3. RE: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble
(Patsy Ruegg)
4. RE: SPAM-LOW: Re: [Histonet] Unstained Slides (Patsy Ruegg)

----------------------------------------------------------------------

Message: 1
Date: Sun, 4 Jul 2010 10:42:47 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW: [Histonet] Re: replacement Turtox Internal
Ear, cochlea slides
To: "'Atoska Gentry'" <gentras <@t> auburn.edu>,
<histonet <@t> pathology.swmed.edu> Message-ID:
<7568A26877EB4C92BD7F83FD0C984AE6 <@t> prueggihctechlt> Content-Type:
text/plain; charset="us-ascii"

Wow that is going to be a tuff one, back in the day I tried to cut
Petris pyramid from the inter ear and was told it was the
densest/hardest bone in
the body and it sure seemed to be. Even decalcified we had to embed it
in plastic and use permanent tungsten carbide D profile knives to cut
any sections. I am not at the university anymore so can't access those
slides or I would try to help you out.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech 12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060 fax 720-859-4110
www.ihctech.net www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska
Gentry
Sent: Thursday, July 01, 2010 10:24 AM
To: histonet <@t> pathology.swmed.edu
Subject: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear,
cochlea slides

Hello! please see below inquiry from a colleague. Thanks! Atoska

Our histology class is looking for replacements for old Turtox (Guinea
Pig) Internal Ear, cochlea slides and was wondering if anyone knew a
company who supplied these slides or tissue blocks? I don't think it has
to be guinea pig but I haven't been able to locate internal ear slides
from any species. Any help would be appreciated. Thanks!

~~~~~~~~~~~~~~~
Michelle (Shelly) Aono
Histology Technician IV
CVM & AU Staff
Advisory Council Representative
Auburn University
CVM-APP 212 Greene Hall, Auburn, AL 36832
(334) 844-5594

------------------------------

Message: 2
Date: Sun, 4 Jul 2010 10:46:25 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks
To: "'Kim Merriam'" <kmerriam2003 <@t> yahoo.com>, "'Sherwood, Margaret'"
<MSHERWOOD <@t> PARTNERS.ORG>, "'Jay Lundgren'" <jaylundgren <@t> gmail.com>,
"'Laurie Colbert'" <laurie.colbert <@t> huntingtonhospital.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8178EEB0DB06440AB8A5250A81E75CE5 <@t> prueggihctechlt>
Content-Type: text/plain; charset="iso-8859-1"

Yep that is what I do, I use the plastic containers I get salad or other
stuff in I would throw away, fill it and freeze it, I add some water to
it and put the blocks on the frozen tray and they get cold and hydrated
at the
same time.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech 12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060 fax 720-859-4110
www.ihctech.net www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim
Merriam
Sent: Thursday, July 01, 2010 6:38 AM
To: Sherwood, Margaret; Jay Lundgren; Laurie Colbert
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks

we use something like this:
https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256&in
E=1&highlight=414004-256

we keep several sizes in the freezer, depending on�how many blocks we
need to cut.� The nice thing is that it is cheap and you can hydrate
your blocks
at the same time you are chilling them.

Kim
�Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

________________________________
From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
To: Jay Lundgren <jaylundgren <@t> gmail.com>; Laurie Colbert
<laurie.colbert <@t> huntingtonhospital.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, June 30, 2010 4:35:53 PM
Subject: RE: [Histonet] Chilling Tray for Blocks

We actually use a styrofoam box (used for shipping) and it stays cold
longer with very little melting.� We keep a couple in the -20 freezer.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jay
Lundgren
Sent: Wednesday, June 30, 2010 4:01 PM
To: Laurie Colbert
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Chilling Tray for Blocks

� � Fill a Tupperware container with water, pop it in the freezer, and
tomorrow you will have a portable block chiller, with the blocks at a
perfect level for your needs, for 1/100th the price.

� � � � � � � � � � � � � � � � � � � � � � � � � � � � Not trying to be
a smarta$$, but....

Jay A. Lundgren, M.S., HTL (ASCP)
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Message: 3
Date: Sun, 4 Jul 2010 10:50:17 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble
To: "'Liz Chlipala'" <liz <@t> premierlab.com>, "'Schneider,Lynda S'"
<emlynda <@t> pathology.ufl.edu>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <068F5447C51843FA9080A848C29DF7D3 <@t> prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"

Oh my, I agree with Liz, your samples are way too thick and your process
is way too short. Even for 3-4mm thickness bone I fix for 24-72 hours
and then
decal in 5% formic acid usually overnight on a platform shaker. I assume
RDO decals a lot faster but not that fast, I do not use RDO because I
think the formic acid which may be slower better preserves the antigens
for IHC.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech 12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060 fax 720-859-4110
www.ihctech.net www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Wednesday, June 30, 2010 5:36 PM
To: Schneider,Lynda S; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble

Overall your fixation, decalcification and processing cycle is too
short. The sample size is really big, why do you need it so thick? I
would first of all let the samples fix longer 24 to 48 hours or I would
trim them so that they are about 3-5 mm in thickness. Either way I like
fixing bone samples for 24 to 48 hours. I'm not that accustomed to
using RDO decal it is hydrochloric acid based and is quicker but even
bone that size would take a while to decal. We normally use a formic
acid based decal and a sample size that large my take up to 2 weeks to
decal. Also to process a sample that size I would be at 4 to 6 hours a
station. We frequently process porcine and goat knees in 2 x 3 blocks
the samples are quite thick about a cm in thickness and we will process
them at 4 to 6 hours a station.

My recommendation would be to decal the larger cube of bone for a period
of time until you can section through it. I would then cut a 3-4 mm
thick piece off the cube and process it at 1 hour per station.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com

Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Schneider,Lynda S
Sent: Tuesday, June 29, 2010 10:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Canine bone sectioning trouble

Hello out there...

We are having trouble sectioning canine bone. The samples are bone
marrow cubes approximately 2cm thick and 1in wide. They were fixed for
15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid
decalcifier. They were faced in and then surface decaled again for
about 30 mins. When sectioned, much of the marrow was missing and the
bone was torn and shredded. We thought that maybe the samples had not
been fixed sufficiently so refixed overnight in 10% NBF again. The
samples were then reprocessed as they had been originally. The
processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH
x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each,
paraffin x 4, 40 mins each. Again they were faced in and decaled for
another 30 mins or so. This time as soon as the sections are placed on
the water bath (38 degrees) they explode and/or come apart so severely
sections can almost not be picked up. If sections are even obtainable
they are of horrible quality. Does anyone have any suggestions? Thank
you so much in advance!

Lynda
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Message: 4
Date: Sun, 4 Jul 2010 10:57:39 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW: Re: [Histonet] Unstained Slides
To: "'Richard Cartun'" <Rcartun <@t> harthosp.org>,
<histonet <@t> lists.utsouthwestern.edu>,
<Rick.Garnhart <@t> memorialhealthsystem.com> Message-ID:
<B54A3617318649AB80325DF13A1C0078 <@t> prueggihctechlt> Content-Type:
text/plain; charset="us-ascii"

I do not bake my unstained slides for IHC controls and store them at
4dc. I
bake them just before use. They do last longer this way but Richard is
right, there is no telling because it all depends on the fixation,
target protein stability and storage conditions. It is always a good
idea to cut
fresh sections if the stored unstained sections do not perform as
expected.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech 12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060 fax 720-859-4110
www.ihctech.net www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, June 28, 2010 10:55 AM
To: histonet <@t> lists.utsouthwestern.edu;
Rick.Garnhart <@t> memorialhealthsystem.com
Subject: SPAM-LOW: Re: [Histonet] Unstained Slides

We bake our unstains at 60 degrees C. for 30 minutes prior to filing at
RT. There is no set rule for stability. It all depends on fixation, the
nature of the protein target, and storage conditions. I've seen
absolutely spectacular immunoreactivity on unstained slides stored at RT
for 20 years
(and longer) and I've seen reduced immunoreactivity in unstained slides
stored for as little as 4 weeks. We will always attempt to stain
unstained slides when available; however, if the lesion or tumor is
negative, and
there is no internal control, you better cut fresh sections.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> <Rick.Garnhart <@t> memorialhealthsystem.com> 6/28/2010 11:54 AM >>>

Histoland, How is everyone storing/filing unstained slide. And how long
are they good for to use for immunohistochemistry.

Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph: 719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> memorialhealthsystem.com

Mission: To provide the highest quality health care
Vision: To create an outstanding health system where patients heal and
people thrive
Values: Compassion - Integrity - Quality - Respect - Teamwork

www.memorialhealthsystem.com

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