[Histonet] TUNEL
Melissa Mazan
melissa.mazan <@t> tufts.edu
Fri Jan 29 11:01:25 CST 2010
Hi all,
I am trying to do an apoptosis assay on mouse lung tissue (FFPE), using
the Trevigen (formerly Roche) TACs TdT system (fluorescent). I am
generating a positive control using nuclease provided by the kit - and
get a beautiful, clean, strong signal. Problem is, when I do the same
assay using the actual tissues, I get no staining whatsoever. I have
included tissues that should have apoptotic cells, including
hyperoxia-damaged lung tissue, neonatal tissue, and post-pneumonectomy
tissue. When I contact the company, they tell me that if my positive
controls are working, then the assay is working, and there is no way to
optomize it further. I have already tried cytonin v. Prot K, and Mn2+
v. Co2+, with no improvement. Biologically, the hyperoxia tissues ought
to be full of apoptotic cells, and all the others should have at least
some. Does anyone have a good protocol or a better kit to suggest?
Thanks! Melissa
histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Ga.Society For Histotechnology Reservation Link
> (Shirley A. Powell)
> 2. Re: Histonet Digest, Vol 74, Issue 34 (Rhonda Henshall-Powell)
> 3. need help with stain (Perry, Margaret)
> 4. Re: need help with stain (Rene J Buesa)
> 5. Thomas Crowell is out of the office. (thomas.crowell <@t> novartis.com)
> 6. RE: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the
> Web - Email found in subject (McCormick, James)
> 7. RE: Re: Diff-Quik (Tony Henwood)
> 8. A blast from the past.... (Jeffrey Silverman)
> 9. (no subject) (Green JumpyOne)
> 10. honing compound (Jennifer MacDonald)
> 11. RE: A blast from the past.... (Mike Pence)
> 12. Re : KP Markers (Vanessa Gutierrez)
> 13. b-5 (Vickroy, Jim)
> 14. Friday Hour of Fun (Breeden, Sara)
> 15. RE: b-5 (Weems, Joyce)
> 16. job opening (Konni Black)
> 17. Decontamination of a Cryostat (Sharon.Davis-Devine)
> 18. Re: Decontamination of a Cryostat
> (Jan.Minshew <@t> leica-microsystems.com)
> 19. RE: Friday Hour of Fun (Mahoney,Janice A)
> 20. Kaposi's sarcoma block (Houston, Ronald)
> 21. RE: Decontamination of a Cryostat (Morken, Tim)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 28 Jan 2010 13:35:48 -0500
> From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
> Subject: [Histonet] Ga.Society For Histotechnology Reservation Link
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9BF995BC0E47744E9673A41486E24EE22429F09B78 <@t> MERCERMAIL.MercerU.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi All,
>
> I wanted to pass this link from the hotel on to you guys who will be attending the Georgia Society for Histotechnology meeting March 26-28 this year. This goes straight to the hotel and already has our code entered for you to reserve your rooms at the Evergreen Marriott Conference Resort. For the complete program and registration form go to www.histosearch.com/gsh<http://www.histosearch.com/gsh> and go to the symposium page. You will find the hotel link there too.
>
> See you there.
>
> Shirley Powell
> GSH Secretary
>
>
>
> Subject: Ga.Society For Histotechnology Reservation Link
>
> Simply cut and paste the link below and include with your electronic correspondence to facilitate the reservation process. Your guests will be directed to the property's home page with the code already entered in the appropriate field. All they need to do is enter their arrival date to begin the reservation process.
>
>
>
>
>
> Evergreen Marriott Conference Resort >><http://www.marriott.com/hotels/travel/atleg?groupCode=gshgsha&app=resvlink&fromDate=3/26/10&toDate=3/27/10>
>
>
>
>
>
> [http://www.marriott.com/Images/Brands/MCC/Logos/MCC_logowhitefield_120x60.gif]<http://www.marriott.com/hotels/travel/atleg?groupCode=gshgsha&app=resvlink&fromDate=3/26/10&toDate=3/27/10>
>
>
>
>
>
> http://www.marriott.com/hotels/travel/atleg?groupCode=gshgsha&app=resvlink&fromDate=3/26/10&toDate=3/27/10
>
>
>
>
>
>
>
>
>
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>
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>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 28 Jan 2010 19:08:33 +0000 (GMT)
> From: Rhonda Henshall-Powell <rlhenshall_powell <@t> yahoo.co.uk>
> Subject: [Histonet] Re: Histonet Digest, Vol 74, Issue 34
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <828544.67370.qm <@t> web23205.mail.ird.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dear Nick,
>
> When I was growing and harvesting 3D cell cultures (spheroids grown in matrigel) we would remove the culture medium, draw up the gel in a needle-less syringe and place in to OCT embedding medium (Tissue Tek) before freezing in Liquid Nitrogen. I would then cut 5-10um sections and perform IHC or immunofluorescence.
>
> Hope this helps - but it looks like you have a lot of good suggestions already.
>
> Best Regards,
> Rhonda
>
> Rhonda Henshall-Powell, Ph.D.
>
>
>> ------------------------------
>>
>> Message: 2
>> Date: Wed, 27 Jan 2010 10:25:42 -0800 (PST)
>> From: Nicholas David Evans <ndevans <@t> stanford.edu>
>>
>> Dear all,
>>
>> I was hoping someone might be able to offer me some advice
>> on embedding and sectioning cell cultures.
>>
>> In short we are growing cells which form 3D dome-like
>> structures on tissue culture plastic. Does anyone have any
>> experience or advice to offer on embedding the cultures in
>> situ before sectioning? I have seen various methods in the
>> literature, which often use Epon to embed the material
>> followed by sawing away the plastic, but if anyone can offer
>> some tips on other possible (easier) ways of doing it, or
>> can refer me to some useful literature, I'd be very
>> grateful.
>>
>> I would like to have simple 10um sections at 90 degrees to
>> the substrate, which I can use for IHC.
>>
>> Best wishes
>> Nick
>>
>>
>>
>> ------------------------------
>>
>> Message: 3
>> Date: Wed, 27 Jan 2010 15:19:36 -0500
>> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
>>
>> Hi Nick:
>>
>> You can use the Epon substitutes such as EmBed 812. Fix,
>> osmicate,
>> dehydrate as usual, but omit the proplylene oxide as it
>> will react with
>> the plastic dish. Epon substitutes will mix with ethanol. I
>> used 2:1
>> then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with
>> catalyst
>> added with agitation. Then several changes of pure Epon and
>> polymerize.
>> Yes, you will have to saw away the plastic, if you try to
>> section the
>> Epon-plastic combo the two will separate.
>> How much easier do you want it to be?
>>
>> Geoff
>> ------------------------------
>>
>> Message: 5
>> Date: Wed, 27 Jan 2010 15:39:05 -0500
>> From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
>> Subject: RE: [HISTONET] embedding cell cultures
>>
>
>
>> Nick,
>>
>> I am assuming that your 3D cells only grow on
>> plastic. They make plastic cover
>> slips which, if your cells attach to them and grow, might
>> make the embedding
>> much easier. You would follow the same method as
>> stated, but then you could
>> invert the coverslips on a beem capsule and separate the
>> coverslip from capsule
>> with liquid nitrogen. However, I have never done it
>> with plastic coverslips
>> (only glass), so not sure if they would easily separate
>> from capsule with liquid
>> nitrogen. If anyone else has done so, please weigh in.
>>
>> Peggy
>>
>> ------------------------------
>> Message: 6
>> Date: Wed, 27 Jan 2010 15:44:37 -0500
>> From: Peggy Bisher <mbisher <@t> Princeton.EDU>
>> Subject: Re: [HISTONET] embedding cell cultures
>>
>> I have done just what you are talking about using Aclar. It
>> is a plastic
>> embedding film (purchased from EMS). It works great for
>> us.
>>
>> Margaret E. Bisher
>> Electron Microscopy & Histology Core Facility Manager
>> Department of Molecular Biology
>> Princeton University
>> Moffett Laboratory, Room 113
>> Princeton, New Jersey
>> Office: (609) 258-7026
>> Fax: (609) 258-8468
>> mbisher <@t> princeton.edu
>>
>
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 28 Jan 2010 15:00:12 -0600
> From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
> Subject: [Histonet] need help with stain
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <FCA5EF47F9BC694CBB4C58FEA04219634CCD07AFC7 <@t> SDSU-MBX.jacks.local>
> Content-Type: text/plain; charset="us-ascii"
>
> A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this?
> Do you have other suggestions that would work better?
> Hope you are warmer than here in SD!
> Thanks for the help.
> Margaret Perry
> South Dakota State University
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 28 Jan 2010 13:21:17 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] need help with stain
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, MargaretPerry
> <Margaret.Perry <@t> sdstate.edu>
> Message-ID: <877399.36977.qm <@t> web65714.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Use mucicarmin stain. The crypt mucus will be evident and their sizes.
> René J.
>
> --- On Thu, 1/28/10, Perry, Margaret <Margaret.Perry <@t> sdstate.edu> wrote:
>
>
> From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> Subject: [Histonet] need help with stain
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, January 28, 2010, 4:00 PM
>
>
> A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this?
> Do you have other suggestions that would work better?
> Hope you are warmer than here in SD!
> Thanks for the help.
> Margaret Perry
> South Dakota State University
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 28 Jan 2010 16:24:40 -0500
> From: thomas.crowell <@t> novartis.com
> Subject: [Histonet] Thomas Crowell is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF24A31EF8.8169811D-ON852576B9.00759D80-852576B9.00759D80 <@t> ah.novartis.com>
>
> Content-Type: text/plain; charset=US-ASCII
>
>
> I will be out of the office starting 01/28/2010 and will not return until
> 02/02/2010.
>
> Please contact Kelly Miner at 617-871-5122 if you have any questions
> regarding clinical trial samples.
>
> ------------------------------
>
> Message: 6
> Date: Thu, 28 Jan 2010 15:29:09 -0600
> From: "McCormick, James" <JMcCormick <@t> schosp.org>
> Subject: RE: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the
> Web - Email found in subject
> To: Robert Richmond <rsrichmond <@t> gmail.com>,
> "Histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0CA58D7DD405854899D129A03276B1334439313EA3 <@t> EXCHCCRMB.schosp.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Samuri.........
> The website www.scienceheritagelimited.com has available 8 books that are reprints of historically important "history of microscopy and histotechnology books" included is the 1885 edition of Arthur Bolles Lee's "The Microtomist's Vade-Mecum". It's amazing how little (relatively) things have changed. The only thing missing in the 1885 edition is the use of formalin as a tissue fixative. This did not come about until 1892 and undoubtedly appears in his later editions.
> Good reading,
>
> jim
> J.B.McCormick, M.D.
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
> Sent: Thursday, January 28, 2010 7:42 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the Web - Email found in subject
>
> Arthur Bolles Lee's The Microtomist's Vade-Mecum, 7th edition (1913)
> is available online on Google Books, along with some other editions:
>
> http://books.google.com/books?id=4m5MAAAAMAAJ&printsec=frontcover&dq=arthur+bolles+lee&ei=BpJhS96xKJTIzASV9vnNBQ&cd=1#v=onepage&q=&f=false
>
> This marvelous old histology recipe book was a great rarity before the
> Web, though it's actually now available from Amazon. It took me
> several years to find a copy in 1976 - found one in a consignment of
> old books discarded from the library of a long-defunct college.
>
> A note about the title: Vade-Mecum (pronounced something like
> voddy-make-um) - literally "come with me" (think of "Quo Vadis) is an
> old word for a practical handbook of something.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
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> Thank you for your cooperation.
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 29 Jan 2010 09:21:55 +1100
> From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] Re: Diff-Quik
> To: <Kim.Donadio <@t> bhcpns.org>, "Robert Richmond" <rsrichmond <@t> gmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID: <B9EAF61856077F47BF9BE2F89AFC555206853866 <@t> hedwig.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Please don't take it personally.
>
> If we are trying our best to meet our own high expectations then we do
> not have to worry.
> I believe that most of us do what Robert expects. This is supported by
> my experiences at the recent NSH meeting in Birmingham Alabama. I was
> privileged to meet some of the most dedicated professionals around and
> believe that our profession is heading in the right direction.
>
> Robert's comments should be heeded and pondered. I definitely did.
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Kim.Donadio <@t> bhcpns.org
> Sent: Thursday, 28 January 2010 2:53 AM
> To: Robert Richmond
> Cc: histonet <@t> lists.utsouthwestern.edu;
> histonet-bounces <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Re: Diff-Quik
>
>
> I'm going to have to agree with Cheryl on the comment. This may be your
> experience but I can tell you my techs always look at their stains
> before
> they send it on to the Pathologist. It is a requirement that they
> understand what they are looking at in order to know if it worked. Each
> of
> them are also trained to know all tissues microscopically and all stain
> components microscopically. That is after all the purpose of being a
> Histologist.
>
> I am going out on a limb here and I normally don't, but you are digging
> yourself in to a rather rude hole to insult so many professional
> Histologist.
>
> Just saying.............
>
>
> Kim Donadio
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
>
>
>
> Robert Richmond <rsrichmond <@t> gmail.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 01/26/2010 07:38 PM
>
> To
> "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> cc
>
> Subject
> [Histonet] Re: Diff-Quik
>
>
>
>
>
>
> I sort of apologize for this ill-natured comment, which long-term
> readers of Histonet know I've made before.
>
> I do locum tenens work, mostly in rather small pathology services - I've
> worked in perhaps 60 of them in my life. Only rarely do I observe that a
> histotech ever looks at a slide. I've just acquired a new client with
> particularly difficult slides. The tech doesn't even have a microscope.
>
> The more quality assurance paperwork I have to do, the worse the slides.
>
> The lack of feedback from pathologist to technologist is a really
> widespread and serious problem. Most pathologists are completely
> unwilling to take the time to do it, and the usage has never established
> itself. It would be much easier if we had double headed microscopes,
> which seem to be prohibited in small pathology services.
>
> Did Edwards Deming live in vain?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> *************************************
> On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com>
> wrote:
>
>> Every slide I stain, special stains, IHC or otherwise I check under
>> the
>>
> scope...I have taught all my techs to do the same, other than batches of
>
> H&E and then we check the 1st slide in each rack. I know this to be a
> common procedure with many histology professionals. The attitude can be
> left in your lab please. Thank you
>
>> Cheryl Miller HT ASCP CM
>> Histology Supervisor
>> Physicians Laboratory Services
>> Omaha, NE. 402 731 4148
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
> Richmond
>
>> Sent: Monday, January 25, 2010 7:50 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Re: Diff-Quik
>>
>> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>>
>> "Diff-Quik" (please note the spelling) is the trademarked name of a
>> staining sequence consisting of a fixative, eosin (Diff-Quik I), and
>> an azure (Diff-Quik II), done in that order in three separate
>> containers. I'm not sure who the trademark presently belongs to - it
>> seems to change with the phases of the Moon.
>>
>> There are a number of generic equivalents, which in my personal
>> experience all work as well as trademark Diff-Quik. For most ordinary
>> pathology services, it isn't worthwhile to try to brew your own.
>>
>> I don't think I've seen bone marrow stained with such a sequence.
>> Proper staining of bone marrows requires that the histotechnologist
>> examine the slides under a microscope, a practice too many find
>> abhorrent.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Knoxville TN
>>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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>
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> ------------------------------
>
> Message: 8
> Date: Thu, 28 Jan 2010 14:53:25 -0800 (PST)
> From: Jeffrey Silverman <pathmaster <@t> yahoo.com>
> Subject: [Histonet] A blast from the past....
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <276280.46298.qm <@t> web111112.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dr Richmond,
>
> Thanks so much for that link to Vade Mecum. I've never before seen the book, but it has brought me back to my childhood, when I found a similar histotechnique book, Michael Guyer's Animal Micrology, in an antique shop my mom was browsing, some 44 years ago. This book was referenced all over that book, and in Gray's Microtomist's Formulary and Guide which I obtained later as my interest in histotechnique grew during high school, an interest kindled by that book and resulting in a rewarding and fascinating career. I feel like a kid again. Very cool.
>
> It's amazing how much mercury they slung around back then. Hell, I made my own Zenker's fluid in high school from those books, bet the waste went down the drain too.
>
> Jeff Silverman
>
> ------------------------------
>
> Message: 9
> Date: Thu, 28 Jan 2010 16:38:59 -0800
> From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
> Subject: [Histonet] (no subject)
> To: <aga8ton <@t> live.com>, <histonet <@t> lists.utsouthwestern.edu>,
> <jkbrown2986 <@t> hotmail.com>
> Message-ID: <BAY107-W2800A82C7D44A6EA4E39D3AB5B0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> http://www.nesle2005.republika.pl/T6vJjAE12x.html
> _________________________________________________________________
> Hotmail: Trusted email with powerful SPAM protection.
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>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 28 Jan 2010 22:03:34 -0800
> From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Subject: [Histonet] honing compound
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF5CAB6073.428DB178-ON882576BA.00211321-882576BA.00214B02 <@t> mtsac.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> A colleague from a neighboring university is trying to find both fine and
> coarse honing compound for his knife sharpener. Does anyone have a good
> source?
>
> Thank you,
> Jennifer MacDonald
>
> ------------------------------
>
> Message: 11
> Date: Fri, 29 Jan 2010 08:13:30 -0600
> From: "Mike Pence" <mpence <@t> grhs.net>
> Subject: RE: [Histonet] A blast from the past....
> To: "Jeffrey Silverman" <pathmaster <@t> yahoo.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <661949901A768E4F9CC16D8AF8F2838C017A3D28 <@t> is-e2k3.grhs.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> How true!.... Can you believe the things we use to put down the drain or in the trash? "Out of sight, out of mind". Today a high chemistry lab would be shut down and the teacher sent to jail for some of the things they taught us back then.
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman
> Sent: Thursday, January 28, 2010 4:53 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] A blast from the past....
>
>
> Dr Richmond,
>
> Thanks so much for that link to Vade Mecum. I've never before seen the book, but it has brought me back to my childhood, when I found a similar histotechnique book, Michael Guyer's Animal Micrology, in an antique shop my mom was browsing, some 44 years ago. This book was referenced all over that book, and in Gray's Microtomist's Formulary and Guide which I obtained later as my interest in histotechnique grew during high school, an interest kindled by that book and resulting in a rewarding and fascinating career. I feel like a kid again. Very cool.
>
> It's amazing how much mercury they slung around back then. Hell, I made my own Zenker's fluid in high school from those books, bet the waste went down the drain too.
>
> Jeff Silverman
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 29 Jan 2010 06:41:34 -0800 (PST)
> From: Vanessa Gutierrez <vygutz <@t> yahoo.com>
> Subject: [Histonet] Re : KP Markers
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <738912.31896.qm <@t> web30206.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> KP Marker pens are sold by Mercedes Medical. I use them and I really like them. The box I ordered is still on back order so you want to order as soon as possible.
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Fri, 29 Jan 2010 09:21:49 -0600
> From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> Subject: [Histonet] b-5
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <24A4826E8EF0964D86BC5317306F58A54256B2E3FA <@t> mmc-mail.ad.mhsil.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Someone remind me. (senior moment). Years ago we stopped using B-5 fixative in our lab. If I remember right we were given a date by CAP to either stop using a mercury product or present a plan on when we would stop using it. Am I correct and if not are some people still using B-5?
>
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
> ________________________________
> This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 29 Jan 2010 08:37:24 -0700
> From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
> Subject: [Histonet] Friday Hour of Fun
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4D14F0FC9316DD41972D5F03C070908B02E46E12 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I have a Hypothetical Situation for which I would like your input. If
> you - as a histologist - were to be involved in the interview of a
> potential staff pathologist, what questions would you ask this
> candidate? Think of the practical and logical issues, but also the
> unique and pertinent secondary Things You'd Like to Know. I would like
> input from techs AND pathologists. If you were a pathologist in an
> interview situation, what questions would you think appropriate? If
> you're a tech, what would you like to know about this candidate before
> he begins to orbit your universe?
>
>
>
> Please write me off Histonet - not for any other reason than it's likely
> that everyone will not be interested in this subject. Thank you again
> and I can't wait to see what answers I will get!
>
>
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM 87106
>
> 505-841-2576
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 29 Jan 2010 10:42:08 -0500
> From: "Weems, Joyce" <JWeems <@t> sjha.org>
> Subject: RE: [Histonet] b-5
> To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B36E22177C738742AE407DBA366FFD4A2447F1 <@t> ITSSSXM02V1.one.ads.che.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Shhhhhhhhhhhhhhhhh, if I understand correctly, they are not legal. It
> was several years ago that we were mandated to stop. j
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy,
> Jim
> Sent: Friday, January 29, 2010 10:22
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] b-5
>
>
> Someone remind me. (senior moment). Years ago we stopped using B-5
> fixative in our lab. If I remember right we were given a date by CAP to
> either stop using a mercury product or present a plan on when we would
> stop using it. Am I correct and if not are some people still using
> B-5?
>
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical and Autopsy Pathology Technical Supervisor Memorial Medical
> Center
> 217-788-4046
>
>
> ________________________________
> This message (including any attachments) contains confidential
> information intended for a specific individual and purpose, and is
> protected by law. If you are not the intended recipient, you should
> delete this message. Any disclosure, copying, or distribution of this
> message, or the taking of any action based on it, is strictly
> prohibited.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Confidentiality Notice:
> This email, including any attachments is the
> property of Catholic Health East and is intended
> for the sole use of the intended recipient(s).
> It may contain information that is privileged and
> confidential. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are
> not the intended recipient, please reply to the
> sender that you have received the message in
> error, then delete this message.
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Fri, 29 Jan 2010 08:04:28 -0800
> From: "Konni Black" <kblack <@t> digestivehlth.com>
> Subject: [Histonet] job opening
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <A8E2E669B37846D484A164A185E34B30 <@t> digestivehlth.com>
> Content-Type: text/plain; charset="Windows-1252"
>
> We are looking for a fulltime histologist for a new lab in Olympia, WA. Planned opening in March with great new equipment and lab space. The ideal candidate will have 3 to 5 years experience and must be able to work alone. Flexible work schedule. .
>
> Contact Konni Black
> kblack <@t> digestivehlth.com
> 253-503-2560
>
>
>
> ------------------------------
>
> Message: 17
> Date: Fri, 29 Jan 2010 10:16:16 -0600
> From: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
> Subject: [Histonet] Decontamination of a Cryostat
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5 <@t> EXCHANGEBE2.carle.com>
> Content-Type: text/plain; charset="us-ascii"
>
> This issue seems to be rearing its ugly head in our lab once again.
> What is the correct procedure for decontaminating a cryostat after let's
> say a specimen from an HIV patient for a frozen was cut on it? We have
> a couple of different cryostats, one of them defrosts quickly and the
> other one ices over and defrosts very slowly. Our PA's assist with 98%
> of the frozens and will use either one of the cryostats. Our
> pathologists, who perform frozens by themselves on a rare occasion,
> prefer the cryostat that defrosts very slowly. Of course the cryostat
> that was contaminated was down awaiting decontamination when a
> pathologist had to perform a frozen. We are now being told that we need
> to replace the cryostat that the pathologist doesn't like to use. So
> have any of you had any issues like this and if you have how did you
> handle it? Are there any decontamination procedures available for a
> quick turnaround? Any help or suggestions will be greatly appreciated.
> Thanks.
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology-Histology Supervisor
>
> Carle Foundation Hospital
>
> Laboratory and Pathology Services
>
> 611 West Park Street
>
> Urbana, Illinois 61801
>
> 217-383-3572
>
> sharon.davis-devine <@t> carle.com
>
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Fri, 29 Jan 2010 10:34:29 -0600
> From: Jan.Minshew <@t> leica-microsystems.com
> Subject: Re: [Histonet] Decontamination of a Cryostat
> To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
> Cc: histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFDD417A5F.25423B3D-ON862576BA.0059E70A-862576BA.005B0D08 <@t> leica-microsystems.com>
>
> Content-Type: text/plain; charset=ISO-8859-1
>
>
> Hi Sharon,
>
> I copied and pasted a chemical disinfection procedure for cryostats that I
> created for Leica's sales and service employees and customers. It
> references Leica cryostats but it can be used for any cryostat--the basic
> principles for chemical disinfection are the same. Since we can't put
> attachments on the histonet, I am hoping that you will be able to read the
> copy without problem. If any of you have trouble, please let me know and I
> can send it to you off line as an attachment.
>
>
> Chemical Disinfection of a Cryostat
>
> According to CAP regulations, a cryostat should be defrosted and
> decontaminated with a tuberculocidal disinfectant at a time interval
> appropriate for the institution (once a week for instruments used daily).
> The regulations also state that the cryostat must be clearly marked as
> contaminated if a frozen section is performed on tissue from a patient
> known or suspected to be positive for HIV, hepatitis B or C, SARS-related
> coronavirus, prion disease such as Creutzfeldt-Jakob disease, or
> mycobacterial or systemic fungal disease. It must then be decontaminated
> before further use.
>
> · Wear Personal Protective Equipment (PPE):
> Personal Protective Equipment, such as gowns, puncture and penetration
> resistant gloves and eye protection must be worn when performing cryostat
> disinfection procedures.
>
> · Cryostat Preparation
> Remove used blades/knives from their holder. Although not a requirement,
> steel mesh gloves should be worn when changing knife blades. Dispose of
> blades according to the regulations of the institution or disinfect
> knives before reusing by soaking in disinfecting solution. Remove all
> debris and utensils (pencils, forceps, brushes, gauze, etc) from the
> chamber. Debris must be removed because organic material (blood and
> proteins) may contain high concentrations of microorganisms and could
> possibly inactivate the chemical disinfectant or prevent access to
> contaminated surfaces. It should be treated as biohazardous waste and
> disposed of according to the policies and procedures of the institution.
>
> 70% ethyl or reagent alcohol can be used to clean the cryostat and
> provide some disinfection capabilities. The germicidal activity of ethyl
> alcohol is most effective in the 70% range because it can penetrate
> tubercle bacteria and it has an advantage over isopropyl alcohol of being
> able to kill hydrophilic viruses.
>
> · Chemical Disinfection
> In order to disinfect a cryostat using a chemical disinfectant, the
> instrument MUST be at room temperature before the process is started.
> Turn off and unplug the instrument before beginning the disinfection
> process. (Once the cryostat has reached room temperature, do not turn
> the handwheel until it has been returned to cold temperature.)
>
> Chemical Disinfection of a Cryostat continued:
>
> Do not create aerosols by spraying disinfectant (or anything else) in an
> open cryostat chamber. Pour disinfectants onto surfaces or absorbent
> disposable towels and allow them to remain in contact with contaminated
> surfaces for the length of time specified in the instructions of the
> individual agents. Use a tuberculocidal disinfectant that is
> non-corrosive. The EPA maintains a list of Antimicrobial Chemical/
> Registration Number Indexes on their website,
> http://www.epa.gov/oppad001/chemregindex.htm), and it is updated
> regularly. From this link you can find agents effective against
> bloodborne pathogens such as Mycobacterium tuberculosis, human HIV-1
> virus, and Hepatitis B or Hepatitis C virus. It is critical to remember
> that NONE of these solutions have been tested at low temperatures and can
> only be used at room temperature.
>
> Any disposable material used in the disinfection process must be disposed
> of in accordance with the policies and procedures of the institution.
>
> · Following Disinfection
> After the disinfection procedure is complete, the cryostat and all of the
> accessories must be thoroughly dried and lubricated before being put back
> into service at cold temperatures. Absolute ethyl alcohol can be used to
> remove excess moisture from surfaces. Accessories with multiple parts,
> such as the disposable blade holder, knife holder and their respective
> bases, must be taken apart and dried thoroughly.
>
> Plug the instrument back in and turn it on. Use only the lubricants that
> are recommended by the manufacturer and only in the recommended amounts.
> Lightly lubricate any moving parts. To lubricate the specimen arm,
> extend it all the way forward. Apply ONE DROP of lubricant to the barrel
> and spread it around with your gloved finger. Move the arm back to the
> home position.
>
> The liquid waste container should be emptied in accordance with the
> policies and procedures of the institution. Before replacing in the
> instrument, add a small amount of liquid bleach to the empty container.
>
> For optimum sectioning, allow the instrument to cool long enough to allow
> the metal microtome parts to reach the cold temperature setting. The
> CM1850 requires no less than 3 hours and the CM1950 needs 5 hours to cool
> down from ambient temperature (20°C) to -25°C and 8 hours to cool from
> 20°C to -35°C.
>
>
>
>
>
>
>
> Kind regards,
>
> Jan Minshew
> Marketing Manager
> Leica Microsystems
> Biosystems Division
> 2345 Waukegan Road
> Bannockburn, IL 60015
>
> Office: 847.405.7051
> Cell: 847.970.8468
> Fax: 847.405.6560
>
> www.leica-microsystems.com
>
>
> Click Here for this month's special offers!
> http://www.leica-microsystems.com/bsdspecial
>
>
>
>
> "Sharon.Davis-Dev
> ine"
> <Sharon.Davis-Dev To
> ine <@t> carle.com> <histonet <@t> lists.utsouthwestern.edu>
> Sent by: cc
> histonet-bounces@
> lists.utsouthwest Subject
> ern.edu [Histonet] Decontamination of a
> Cryostat
>
> 01/29/2010 10:16
> AM
>
>
>
>
>
>
>
> This issue seems to be rearing its ugly head in our lab once again.
> What is the correct procedure for decontaminating a cryostat after let's
> say a specimen from an HIV patient for a frozen was cut on it? We have
> a couple of different cryostats, one of them defrosts quickly and the
> other one ices over and defrosts very slowly. Our PA's assist with 98%
> of the frozens and will use either one of the cryostats. Our
> pathologists, who perform frozens by themselves on a rare occasion,
> prefer the cryostat that defrosts very slowly. Of course the cryostat
> that was contaminated was down awaiting decontamination when a
> pathologist had to perform a frozen. We are now being told that we need
> to replace the cryostat that the pathologist doesn't like to use. So
> have any of you had any issues like this and if you have how did you
> handle it? Are there any decontamination procedures available for a
> quick turnaround? Any help or suggestions will be greatly appreciated.
> Thanks.
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology-Histology Supervisor
>
> Carle Foundation Hospital
>
> Laboratory and Pathology Services
>
> 611 West Park Street
>
> Urbana, Illinois 61801
>
> 217-383-3572
>
> sharon.davis-devine <@t> carle.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ______________________________________________________________________
> This email has been scanned by the MessageLabs Email Security System.
> For more information please visit http://www.messagelabs.com/email
> ______________________________________________________________________
>
>
>
> ------------------------------
>
> Message: 19
> Date: Fri, 29 Jan 2010 10:44:04 -0600
> From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
> Subject: [Histonet] RE: Friday Hour of Fun
> To: "'Breeden, Sara'" <sbreeden <@t> nmda.nmsu.edu>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A562C <@t> EXCHMBC2.ad.ah.local>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I'd ask how he sees his relationship with the histotechnologists, histo supervisor, etc.
> I'd ask him what his expectation is regarding TAT of slides/special stains/IHC/rectus.
> If there is more than one Pathologist, I'd ask how they would collaborate to decide on standard work. For example, will you cut one two or three slides on GI bxs. (in other words will he accept that you will not do specific things for individual Pathologists, that they will decide on standard work).
> these are a few thing off the top of my head.
> Good luck, it will be interesting to see what others come up with.
> Jan Mahoney
> Omaha, NÉE
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
> Sent: Friday, January 29, 2010 9:37 AM
> To: histonet
> Subject: [Histonet] Friday Hour of Fun
>
> I have a Hypothetical Situation for which I would like your input. If
> you - as a histologist - were to be involved in the interview of a
> potential staff pathologist, what questions would you ask this
> candidate? Think of the practical and logical issues, but also the
> unique and pertinent secondary Things You'd Like to Know. I would like
> input from techs AND pathologists. If you were a pathologist in an
> interview situation, what questions would you think appropriate? If
> you're a tech, what would you like to know about this candidate before
> he begins to orbit your universe?
>
>
>
> Please write me off Histonet - not for any other reason than it's likely
> that everyone will not be interested in this subject. Thank you again
> and I can't wait to see what answers I will get!
>
>
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM 87106
>
> 505-841-2576
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person.
>
> The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation.
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Fri, 29 Jan 2010 11:45:00 -0500
> From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
> Subject: [Histonet] Kaposi's sarcoma block
> To: <ihcrg <@t> googlegroups.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <979FF5962E234F45B06CF0DB7C1AABB21B646032 <@t> chi2k3ms01.columbuschildrens.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Can anyone spare a block of Kaposi's sarcoma?
>
> Thanks
>
>
>
> Ronnie Houston, MS HT(ASCP)QIHC
>
> Anatomic Pathology Manager
>
> ChildLab, a Division of Nationwide Children's Hospital
>
> www.childlab.com
>
> 700 Children's Drive
>
> Columbus, OH 43205
>
> (P) 614-722-5450
>
> (F) 614-722-2899
>
> ronald.houston <@t> nationwidechildrens.org
>
> www.NationwideChildrens.org <http://www.NationwideChildrens.org>
>
>
>
> "One person with passion is better than forty people merely interested."
> ~ E.M. Forster
>
>
>
>
>
> ----------------------------------------- Confidentiality Notice:
> The following mail message, including any attachments, is for the
> sole use of the intended recipient(s) and may contain confidential
> and privileged information. The recipient is responsible to
> maintain the confidentiality of this information and to use the
> information only for authorized purposes. If you are not the
> intended recipient (or authorized to receive information for the
> intended recipient), you are hereby notified that any review, use,
> disclosure, distribution, copying, printing, or action taken in
> reliance on the contents of this e-mail is strictly prohibited. If
> you have received this communication in error, please notify us
> immediately by reply e-mail and destroy all copies of the original
> message. Thank you.
>
> ------------------------------
>
> Message: 21
> Date: Fri, 29 Jan 2010 08:45:45 -0800
> From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: [Histonet] RE: Decontamination of a Cryostat
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1AAF670737F193429070841C6B2ADD4C0121C3B1D8 <@t> EXMBMCB15.ucsfmedicalcenter.org>
>
> Content-Type: text/plain; charset=us-ascii
>
> Cryostat decontamination references:
>
>
> Decontamination for HIV
> Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J Histotechnol, V9, No.1, p.29 (March 1986)
> Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV).
>
>
> College of American Pathologists, Commentary on accreditation questionaire:
> 1) Decontaminate regularly with 70% alcohol.
> 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily
> 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997
> http://www.cap.org/HTML/checklist_html/cklst08p.html
>
> NCCLS site: http://www.nccls.org/
>
>
>
> Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee.
> Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer).
>
> Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994.
> Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given).
>
> For those who like to read a lot:
> APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each.
> Tim Morken
> Supervisor, Histology / IPOX
> UCSF Medical Center
> San Francisco, CA
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine
> Sent: Friday, January 29, 2010 8:16 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Decontamination of a Cryostat
>
> This issue seems to be rearing its ugly head in our lab once again.
> What is the correct procedure for decontaminating a cryostat after let's
> say a specimen from an HIV patient for a frozen was cut on it? We have
> a couple of different cryostats, one of them defrosts quickly and the
> other one ices over and defrosts very slowly. Our PA's assist with 98%
> of the frozens and will use either one of the cryostats. Our
> pathologists, who perform frozens by themselves on a rare occasion,
> prefer the cryostat that defrosts very slowly. Of course the cryostat
> that was contaminated was down awaiting decontamination when a
> pathologist had to perform a frozen. We are now being told that we need
> to replace the cryostat that the pathologist doesn't like to use. So
> have any of you had any issues like this and if you have how did you
> handle it? Are there any decontamination procedures available for a
> quick turnaround? Any help or suggestions will be greatly appreciated.
> Thanks.
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology-Histology Supervisor
>
> Carle Foundation Hospital
>
> Laboratory and Pathology Services
>
> 611 West Park Street
>
> Urbana, Illinois 61801
>
> 217-383-3572
>
> sharon.davis-devine <@t> carle.com
>
>
>
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>
>
>
> ------------------------------
>
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>
> End of Histonet Digest, Vol 74, Issue 36
> ****************************************
>
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel: 508-839-5395
fax: 508-839-7903
email: melissa.mazan <@t> tufts.edu
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