[Histonet] need help with stain

[Rene J Buesa]

Use mucicarmin stain. The crypt mucus will be evident and  their sizes.
René J.

--- On Thu, 1/28/10, Perry, Margaret <Margaret.Perry <@t> sdstate.edu> wrote:


From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] need help with stain
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, January 28, 2010, 4:00 PM


A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use.  I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane.  What is your opinion on this? 
Do you have other suggestions that would work better?
Hope you are warmer than here in SD!
Thanks for the help.
Margaret Perry
South Dakota State University



















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