[Histonet] Coverslipping

[John Kiernan]

Is it "normal" to put the mounting medium on the slide and then apply a coverslip from above? What's wrong with putting the mounting medium on the supine coverslip and then bringing the slide down from above?  I prefer the latter method for preparations thinner than 50um.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Date: Tuesday, January 26, 2010 14:13
Subject: [Histonet] Coverslipping
To: histonet <histonet <@t> lists.utsouthwestern.edu>

> Do any of my Esteemed Colleagues have a photo somewhere of someone
> hand-coverslipping?  I need a picture of a "normal" hand 
> coverslipping(putting coverslip ON slide instead of slide ON 
> coverslip).  Just one
> photo is all I need.  I'm trying to describe in words how 
> to do this and
> it's not easy!  Thanks in advance for your Digital Donation 
> (and I mean
> "electronic" digital, so don't give me any flak).  If you 
> can email it,
> I can pass it along.
> 
>  
> 
> Sally Breeden, HT(ASCP)
> 
> NM Dept. of Agriculture
> 
> Veterinary Diagnostic Services
> 
> PO Box 4700
> 
> Albuquerque, NM  87106
> 
> 505-841-2576
> 
>  
> 
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