[Histonet] Wright-Giemsa stain

[Marshall, Kimberly]

I am from a small Hospital where due to the size, Histology does the
Bone Marrow's.  We go to the procedure, make the smears, as well as do
the Wright-Giemsa stain.   I am having a hard time getting the Wright's
stain dark enough for my Pathologist.   I have added stain time, checked
the pH of my stain, tried different rinse's and buffers, but still not
getting it dark enough.  I do go back to the stain for 45 mins or more
and that will get it at least close to what they want.  Is there anyone
out there that can give me some advise on this stain?   Thanks in
advance for your time

 

Kimberly


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