[Histonet] Movats on mouse aortas

[Molinari, Betsy]

Hi,

 I have had a problem with "splotchy" elastin staining on certain
sections of mouse aorta. I use the Modified Movats and do not use a
microwave. But from this one lab  some areas of the elastic fibers will
retain the hematolylin after differentiation while some areas lose it
completely, all in the same section. 

 They fix in 10%NBF and the processing gets done at their lab and I
receive the unstained slides. My control does not have this problem.

Any ideas?

Thanks,

 

Betsy Molinari HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812