[Histonet] top off eosin with alcohol

[Mike Pence]

I always top with the next alcohol in the line and then rotate the alcohols. This refreshes the eosin and gives you a cleaner line.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Saturday, January 16, 2010 8:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] top off eosin with alcohol



when eosin evaporates, the dye concentration increases and adding more eosin will increase your stain strength. Top with a good quality (not recycled) alcohol to maintain the level.  
 
This is assuming the level drops due to evaporation, not carry-over.
 
Cheryl
 
 
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