Re: Re: [Histonet] 0.1 M PB & 0.01M PBS

[TF]

For longer term preservation of brain sectins in floating state...0.01M PBS is not as good as 0.1 M PB..


2010-01-18 



TF 



发件人： Adam . 
发送时间： 2010-01-18  11:02:26 
收件人： tifei 
抄送： Histonet <@t> lists.utsouthwestern.edu 
主题： Re: [Histonet] 0.1 M PB & 0.01M PBS 
 
I've used 1X PBS for everything and it seems to work.

Adam


On Sun, Jan 17, 2010 at 8:41 PM, TF <tifei <@t> foxmail.com> wrote:

We use 0.1 PB for tissue fixation (4% PFA), cryo-preservation (30% sucrose)
then go 0.01 PBS for IHC

though these are routines here, I wonder anyone can specify on the use of two different solutions, especially why?


2010-01-18



TF
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet