[Histonet] Re: Mitochodrial IHC marker in FFPE skeletal

[Amos Brooks]

Hi,
   Regarding mitochondrial IHC SDHB works well in paraffin sections. I used
the one from AbCam.

Amos



Date: Fri, 15 Jan 2010 19:47:31 +0000
From: "Hobbs, Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] Re: Mitochodrial IHC marker in FFPE skeletal
       muscle
To: "histonet <@t> lists.utsouthwestern.edu"
       <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
       <
11D9615B89C10747B1C985966A63D7CA2C434CE92F <@t> KCL-MAIL04.kclad.ds.kcl.ac.uk>

Content-Type: text/plain; charset="us-ascii"


I have not yet found an anti mitochondrial Ab that works in mouse FFPE
tissues, so I am very interested.
However, the protein that Chemicon's MAB1273 is directed against
survives....it has worked very well for me in human FFPE sections after
Citric acid HIER ( see here for images:
http://www.immunoportal.com/index.php )
At light microscopic level, no mitochondrial architecture is visible:
rather, a series of dots are seen.

STAT3 in mitochondria?

Good luck.
Sincerely,
carl