[Histonet] RE: Experience with Tissue Tek VIP6 processor

[Nancy Klemme]

Hello, Dr. Rabe:

I working on my 40th year as a histologist and remember hearing or reading of tissue processing by going from isopropanol directly into the paraffin step that had taken place in the 1950's or '60's.  I'm not sending this as a declaration of my age, but, rather as a reference that xylene-free processing has been around for a long, long time, even though most of us used a clearing step between the dehydrant and the paraffin steps.  ANY tissue processor (human or mechanical) can be used for this technique because the key elements are the chemicals that the tissues are being exposed to.

Because attachments cannot be included, as a private eMail, I will send you a White Paper that Sakura published last year using this technique with the VIP6 that you're considering.  Anyone reading this may obtain it from your Sakura Sales Manager or simply send me a return eMail.  I'll also request to have it placed on our company web-site.  As with any change in specimen handling procedure, you will want to document your validation of this technical change.

Whatever instrument you ultimately choose to purchase, I send my kind regards.
Nancy Klemme
Edu. Svcs. Dir. / Sakura Finetek USA, Inc.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe
Sent: Friday, January 15, 2010 8:05 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Experience with Tissue Tek VIP6 processor

Our lab is in the market for a new processor.  Does anyone have experience with the Tissue Tek
VIP 6?  And does anyone have experience using it xylene-free?  Our histology supervisor would
love to talk with you.

Kim Rabe, M.D.
North Central Pathology, P.A.

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