[Histonet] re; eosin

[Sherwood, Margaret]

I never noticed if ours shakes to rid the excess, but I do know the 95% alcohol
after eosin is pink but even when we stained manually, we had that as well.
What we do with the XL is rotate the 2nd 95% to that position and put fresh 95%
in the 2nd position.  However, we only do that daily, not after each run.  Even
manually, we made that switch daily, not after each run.   

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Friday, January 15, 2010 1:04 PM
To: histonet
Subject: [Histonet] re; eosin

I have had some great suggestions from many of you, the problem is I am doing
literally all of them. My question is on the Leica XL isn't  the instrument
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it
is keeping a lot of carryover because it isn't vibrating. Also the alcohol after
eosin is pretty pink after only a few racks. Tell me what you
think.......thanks, Cheri

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

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