[Histonet] RE: Eosin evaporation.

[Sheila Haas]

Cheri,
How close on your stain line is your eosin in relation to the oven?
It's possible if it nearby, the oven is evaporating your eosin more
rapidly than expected. Just a thought.
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories
 




________________________________
From: Cheri Miller <cmiller <@t> physlab.com>
To: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>; "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>; "Mahoney, Janice A" <Janice.Mahoney <@t> alegent.org>; histonet <histonet <@t> lists.utsouthwestern.edu>; "histonet-bounces <@t> lists.utsouthwestern.edu" <histonet-bounces <@t> lists.utsouthwestern.edu>
Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.

We do this as well, that is why I am stumped.

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

-----Original Message-----
From: Sherwood, Margaret [mailto:MSHERWOOD <@t> PARTNERS.ORG]
Sent: Friday, January 15, 2010 9:53 AM
To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.



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