[Histonet] RE: Eosin evaporation.

[Sherwood, Margaret]

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.

Peg 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, January 15, 2010 10:22 AM
To: Mahoney,Janice A; Cheri Miller; histonet;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but that's just me!

Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM  87106
505-841-2576


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