[Histonet] flattening tissue surface

[Rene J Buesa]

Cut very thin (about 2 µm) and let the sections expand well before taking them from the water bath.
After that shake them and put the slides vertical on their smaller side (1 inch) for 15 minutes.
Take to the oven at 60ºC for 10 minutes and proceed as usual.
You could also buy a Kurabo S200 sectioning robot that has been found to be specially useful for the type of work you are describing (better than manual sectioning).
René J. 

--- On Mon, 1/11/10, Karen Cai <kcai <@t> prosci-inc.com> wrote:


From: Karen Cai <kcai <@t> prosci-inc.com>
Subject: [Histonet] flattening tissue surface
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 1:54 PM



Hi,
I need help to prepare some paraffin tissue slides for confocal
microscope evaluation. We need the tissue surface very flattened, no
wrinkle in order that the microscope can focus the whole section evenly.

Anyone has suggestions?

Thanks in advance,

Best,
Karen

Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
www.prosci-inc.com




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