[Histonet] GUS staining on parifin thin sections, or infiltration os stain into developing embryos

[Michelle Jamison]

Hi All
I have been trying to stain for GUS expression (X-Gluc) in developing seeds of Arabidopsis.  I have good expression on my positive control (a "housekeeping gene) except for the developing embryos!  And forget about those samples with the specific gene in the embryos.  One of the things I will be trying is thin sectioning then staining.  Possibly the enzyme will be compromised?  
Does anyone have experience with this sort of thing? 
Please let me know your thoughts and or experiences.
Thank you
Michelle

 

Michelle Jamison
Cytology 
Caisson labs
1740 Research Parkway
North Logan Utah
84341
435.755.7617 ext 109