[Histonet] RE: Superfrost plus gold slides (Sherwood, Margaret )

[Thurby, Christina]

Peggy,
In response to your question, the superfrost plus gold slides do work really well with FFPE tissues.  Under a separate e-mail I'll send you a paper that our laboratory completed using this type of slide (superfrost plus gold vs. superfrost plus) for immuno work involving the brain and gallbladder using animal tissue.
Christina Thurby
Bristol Myers Squibb
phone 812-307-2093

>Message: 13
>Date: Tue, 5 Jan 2010 10:51:21 -0500
>From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
>Subject: Re:  [Histonet] Superfrost plus gold slides
>To: <histonet <@t> lists.utsouthwestern.edu>


>We are staining 5um FFPE sections of laser-treated human cartilage and are
>experiencing sections falling off the slides and, if not, then wrinkling.  (We
>even had this problem with untreated cartilage, but it is worse with
>laser-treated).
>
>I know this topic has been discussed before and went to the Histonet archives,
>but did not get a clear answer to my query.  Most of the discussion re:
>superfrost plus gold slides involved frozen sections, but I did not get a clear
>answer re:  paraffin-embedded sections.
>
>We are currently using superfrost plus slides.  I have been looking in the
>literature re:  coated slides, but it is confusing.  What do most histonetters
>recommend?  (i.e. poly-l-lysine, gelatin, or silane coated
>Slides).  Do you recommend superfrost plus gold slides?
>
>I would appreciate any advice.
>
>Thanks!
>Peggy
>
>
>Peggy Sherwood
>Lab Associate, Photopathology
>Wellman Center for Photomedicine (EDW 214)
>Massachusetts General Hospital
>55 Fruit Street
>Boston, Massachusetts 02114-2696
>617-724-4839 (voice mail)
>617-726-6983 (lab)
>617-726-1206 (fax)
>msherwood <@t> partners.org

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