[Histonet] Problems with Denatured Alcohol?

[Joe Nocito]

Pam is correct, I mean, look at me, I've been denatured for many years.
Happy New Year everyone

JTT
----- Original Message ----- 
From: "Pamela Marcum" <mucram11 <@t> comcast.net>
To: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, January 04, 2010 9:52 AM
Subject: Re: [Histonet] Problems with Denatured Alcohol?




Wanda,



The first thing you need to find out is what did they denature it with. 
Denatured alcohol may not be the mixture of isopropanol and methanol in 
ethanol you were getting and could be chemically denatured.



This is a problem that comes up when we don't check all the variables in 
what is actually in the products we buy and the vendor doesn't tell us why 
it got cheaper. If it is a chemical denaturing of the alcohol it could be 
designed for use in one of many fields just not histology. The real 
requirement for reagent and denatured alcohol with the ATF is that the 
product be undrinkable or unfit for human consumption. Also different 
manufacturers use varying amounts of isopropanol/methanol/ethanol and that 
can change the way it processes and stains tissue. The best is 5% each of 
isopropanol and methanol with the remaining 90% ethanol.



Pam Marcum

UAMS


----- Original Message ----- 
From: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, January 4, 2010 9:25:08 AM GMT -06:00 US/Canada Central
Subject: [Histonet] Problems with Denatured Alcohol?

Happy New Year to Everyone!!!!!
We recently changed from Reagent Alcohol to Denatured Alcohol as a cost 
saving measure per the "bean counters". The cutting histotechs have started 
complaining that the tissue seems dry and they are having difficulty cutting 
sections. Has anyone else had this problem and are there any adjustments in 
processing times, etc that may help the situation???
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax


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