[Histonet] Decaling mouse joints

[Liz Chlipala]

We use formic acid (10%) for decalcification.  We fix in 10% NBF for 48
hours.  We decal for about 7 days for the mouse joints (ankles, front
and rear paws), knees do not take as long.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Igor
Deyneko
Sent: Monday, January 04, 2010 12:17 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decaling mouse joints

Dear Histonetters!
I have a project coming up which would require to decalcify mouse hind
legs,
femur and tibia with joints. Can anyone suggest a good system, either an
ion
exchange or acid decal(formic acid I've heard works well) and also if
anyone
has a protocol on fixation and decaling, it would be much appreciated.
Thank you.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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