[Histonet] special coloration for fish gill chloride cells
Jamshid Amiri Moghaddam
j_amiri <@t> modares.ac.ir
Fri Feb 26 13:48:20 CST 2010
Hello friends
I have some gill tissues for chloride cells localization.
Do you know a method for chloride cells coloration except
immunohistochemical using NKA antibody???
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, February 26, 2010 9:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 75, Issue 37
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Today's Topics:
1. NBS/NIST Thermometers (thisisann <@t> aol.com)
2. Control slides for Flag-Tag IHC (Randolph-Habecker, Julie)
3. Control tissue for Fluoro Jade (Van Fleet, Jonilyn (J))
4. RE: NBS/NIST Thermometers (McMahon, Loralee A)
5. Re: Snap Freezing Tissue (Robert Richmond)
6. voice recognition (Mahoney,Janice A)
7. haematoxylin (Hana Peter)
8. Re: haematoxylin (John Kiernan)
9. Re: shandon's formal fixx and cytoblock kit (shehnaz khan)
10. RE: [ SOLVED ][ Histonet ] Strange circles in IHC slides
(Hoekert, W.E.J.)
11. Fire in the lab (CHRISTIE GOWAN)
12. Re: Fire in the lab (DKBoyd <@t> chs.net)
13. Re: [ SOLVED ][ Histonet ] Strange circles in IHC slides
(Alexandra Meinl)
14. Saturday Tech Needed Orange County California (Paula Lucas)
15. ruo antibodies (Vickroy, Jim)
16. RE: Fire in the lab (Stephanie Rosenwinkel)
----------------------------------------------------------------------
Message: 1
Date: Thu, 25 Feb 2010 13:59:23 -0500
From: thisisann <@t> aol.com
Subject: [Histonet] NBS/NIST Thermometers
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CC8463C76E2CBF-55EC-12CB <@t> webmail-m063.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Does anyone know how frequently you have to certify your NIST Thermometers
(the thermometer you use to calibrate all of the thermometers in the
laboratory), including a reference.
The certifiicate for my NIST Thermometer does not document the next time it
needs to be calibrated.
Thank you,
Ann Angelo
------------------------------
Message: 2
Date: Thu, 25 Feb 2010 11:13:38 -0800
From: "Randolph-Habecker, Julie" <jhabecke <@t> fhcrc.org>
Subject: [Histonet] Control slides for Flag-Tag IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <040346FA7309BD439C327F97D4C4D69B072C778B <@t> ISIS.fhcrc.org>
Content-Type: text/plain; charset="us-ascii"
Folks,
I am looking for a control for some Flag-tag IHC I am doing on FFPE
tissue. I was wondering if someone could share some slides off of a
block of flag transfected cells.
THANKS!!
Julie
Julie Randolph-Habecker, Ph.D.
Staff Scientist - Director
Experimental Histopathology Shared Resource
Fred Hutchinson Cancer Research Center
1100 Fairview Ave, N. DE-360 (Please note new location)
Seattle WA 98109-1024
206-667-6119
jhabecke <@t> fhcrc.org
------------------------------
Message: 3
Date: Thu, 25 Feb 2010 15:44:21 -0500
From: "Van Fleet, Jonilyn (J)" <JVanFleet <@t> dow.com>
Subject: [Histonet] Control tissue for Fluoro Jade
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<11DEE9B567C45C42AEC620F3B836D53C0325E4C4 <@t> USMDLMDOWX027.dow.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonet,
I am looking for a positive control for the Fluoro Jade Stain consisting
of rodent brain tissue demonstrating neuronal necrosis in the
hippocampus caused by administration of trimethyltin. Does anyone have
access to a stock of controls or can anyone tell me who to contact. I
have contacted the AFIP and NSH but neither were able to help. Any
information would be greatly appreciated.
Thank-you,
Jonilyn Van Fleet, HT (ASCP)
The Dow Chemical Company
989-636-3539
jvanfleet <@t> dow.com
------------------------------
Message: 4
Date: Thu, 25 Feb 2010 16:32:35 -0500
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] NBS/NIST Thermometers
To: "thisisann <@t> aol.com" <thisisann <@t> aol.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C27AA2A01CEF31469813089E226F582E63759BEF <@t> urmcms7.urmc-sh.rochester.edu>
Content-Type: text/plain; charset="us-ascii"
I am in NY State and they just told me yesterday that we need to do it
annually.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of thisisann <@t> aol.com
[thisisann <@t> aol.com]
Sent: Thursday, February 25, 2010 1:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] NBS/NIST Thermometers
Does anyone know how frequently you have to certify your NIST Thermometers
(the thermometer you use to calibrate all of the thermometers in the
laboratory), including a reference.
The certifiicate for my NIST Thermometer does not document the next time it
needs to be calibrated.
Thank you,
Ann Angelo
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 25 Feb 2010 16:50:42 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Snap Freezing Tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a61002251350k445c314bif29911bf0dd32d6f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
In the last two or three years I and others have posted a number of
times on Histonet about the Histobath (which is no longer in
manufacture), Bright's Clini-RF, and 3M's NovecT Engineered Fluid
HFE-7100. You can access these posts in the Archives, through Google
if you wish.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 6
Date: Thu, 25 Feb 2010 15:55:10 -0600
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: [Histonet] voice recognition
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C49F24 <@t> EXCHMBC2.ad.ah.local>
Content-Type: text/plain; charset="us-ascii"
Hello,
Can anyone using voice recognition with Cerner Millennium tell me what
system they are using and how they like it?
Thanks,
Jan Mahoney
Omaha, NE
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Message: 7
Date: Thu, 25 Feb 2010 23:15:40 +0100
From: Hana Peter <hana444 <@t> gmail.com>
Subject: [Histonet] haematoxylin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4B86F68C.70003 <@t> gmail.com>
Content-Type: text/plain; charset=ISO-8859-2; format=flowed
Hello everyone!
Does anyone know where I can get more information about
haematoxylin-haematein-oxyhaematein chemistry?
I have a problem with my Harris haematoxylin modification (sodium iodate
as oxidant). When I use a smaller amount of sodium iodate (about 0,1g
per 1g of haematoxylin) I have a huge problem with precipitation. I
don't know if this is because of the unoxidized haematoxylin in staining
solution or because of something else.
Any help or suggestions are welcomed!
Thanks in advance!
Peter
------------------------------
Message: 8
Date: Thu, 25 Feb 2010 23:39:27 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] haematoxylin
To: Hana Peter <hana444 <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <fbc5a2c14eb1.4b870a2f <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII
0.1G should give you an approximately half-oxidized hamatoxylin solution
along the lines of Baker's haematal-8 or haematal-16, or Gill's haemalum. It
should keep well. Precipitation must be due to something else you're doing.
For the chemistry a good starting point is Conn's Biological Stains (10th
ed, 2002). for information, see http://biologicalstaincommission.org and
click on "Publications".
The current issue of Biotechnic & Histochemistry (Feb. 2010) is a special
issue devoted to haematoxylin (Vol 85 No. 1). Last year the same journal
(Vol 84 No. 4, pp. 159-177) carried an 18-page paper, "Nuclear staining with
alum hematoxylin" by Bryan Llewellyn, which includes recipes for scores of
haemalum mixtures and has much other interesting information. You can review
the contents of the journal at http://informahealthcare.com/loi/bih. If your
institution's library subscribes to the Informa Healthcare package of
journals, you'll be able to download PDF files of articles - they go right
back to 1925.
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Hana Peter <hana444 <@t> gmail.com>
Date: Thursday, February 25, 2010 17:16
Subject: [Histonet] haematoxylin
To: histonet <@t> lists.utsouthwestern.edu
> Hello everyone!
> Does anyone know where I can get more information about
> haematoxylin-haematein-oxyhaematein chemistry?
>
> I have a problem with my Harris haematoxylin modification
> (sodium iodate as oxidant). When I use a smaller amount of
> sodium iodate (about 0,1g per 1g of haematoxylin) I have a huge
> problem with precipitation. I don't know if this is because of
> the unoxidized haematoxylin in staining solution or because of
> something else.
>
> Any help or suggestions are welcomed!
> Thanks in advance!
> Peter
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Fri, 26 Feb 2010 13:30:41 +0200
From: shehnaz khan <shehnazster <@t> gmail.com>
Subject: [Histonet] Re: shandon's formal fixx and cytoblock kit
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4fe9f16e1002260330j668fa3fdn2f20537f01a75745 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
On Sat, Feb 20, 2010 at 3:54 PM, shehnaz khan <shehnazster <@t> gmail.com> wrote:
> Hi Histonetters
>
> Could someone kindly share their views on the shandon's formal fixx
> (concentrate) and cytoblock kit for cell block preparation in cytology?
Has
> it been effective for cellular preservation and cell capture from
inadeduate
> samples?
>
> Thanking you in advance.
>
> S .Khan
> Dept of Cytology
> University of Witwatersrand
> Johannesburg
> South Africa
>
------------------------------
Message: 10
Date: Fri, 26 Feb 2010 13:44:51 +0100
From: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>
Subject: RE: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC
slides
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>,
<histonet.nospam <@t> vneubert.com>
Message-ID: <1190CB05C44B13409483514729C2FC360C0A98 <@t> PAIT42.olvg.nl>
Content-Type: text/plain; charset="iso-8859-1"
Are you sure that you don't introduce air bubbles when you put your slides
into the coverplates? The antibody will not touch the tissue if there is an
air bubble.
Willem Hoekert
________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Rene J Buesa
Verzonden: do 25-2-2010 16:42
Aan: histonet <@t> lists.utsouthwestern.edu; histonet.nospam <@t> vneubert.com
Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC
slides
To me it seems that the sections after being picked from the water bath were
not completely drained and the dewaxing process was incomplete in a way that
the "round" areas kept certain amount of paraffin wax that prevented the
reagents reactions.
The fact that the areas are round are an indication that water was involved,
since water always leave a round imprint, due to its surface tension.
I would suggest that you dewax the sections with a 2% aq. solution of dish
washer soap.
Dewaxing with xylene sections containing water will be incomplete because it
does not mix completely with water but the detergent will mix with the water
and will better remove the paraffin.Reni J.
--- On Thu, 2/25/10, histonet.nospam <@t> vneubert.com
<histonet.nospam <@t> vneubert.com> wrote:
From: histonet.nospam <@t> vneubert.com <histonet.nospam <@t> vneubert.com>
Subject:>[Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, February 25, 2010, 8:34 AM
Hello Histonet,
it has been a while (~10 months) since I posted a problem about uneven
immuno-staining with specimen showing unstained circles after manual
staining with HRP-polymere/DAB method; complete mail see below, response
mails see Histonet archive (via website).
Links to pictures I took:
http://img12.imageshack.us/img12/8513/ts0402162049.jpg
http://img13.imageshack.us/img13/6514/ts0402162104.jpg
The problem occured suddenly, without having changed any reagents or
methods.
Things I changed to avoid the unstained spots:
*Adding 0,05% Tween 20 to TBS
*Blocking peroxidase in coplin jar, not mounted in racks
*Lowering antibody concentration
which temporarily produced better results.
After reviewing a big number of slides it showed up that most of the tissue
affected was lung, liver and kidney which mostly means a lot of blood in the
tissue when fixation in formalin starts.
Erythrocytes, granulocytes and macrophages show a lot of endogenous and
pseudoendogenous peroxidase activity.
This is how it's done since then:
Slides are taken from racks into a big coplin jar with 3% H2O2 diluted in
distilled water (demineralized H2O).
A slow magnetic stirrer on the bottom of the jar keeps the solution floating
around the tissue, removing any O2 bubble that might appear. Slides then are
remounted and rinsed a lot with TBS-T.
Thank you for all your help, though it's a little late...
V. Neubert,
Germany
----- Original Message -----
From: histonet.nospam <@t> vneubert.com
To: histonet <@t> lists.utsouthwestern.edu
Date: 02.04.2009 18:12:18
Subject: [Histonet] Strange circles in IHC slides
[...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg
> http://img13.imageshack.us/img13/6514/ts0402162104.jpg
[...]>
> So, has ever anyone experienced sth. like this?
> My conjugate control (every step except the antibody) was fine, nothing
> to be seen about DAB and no circles at all.
>
> I used Shandon single-use coverplates, sterile buffer, fresh antibody
> aliquots. Any idea?
>
> Thanks,
>
> V. Neubert
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Message: 11
Date: Fri, 26 Feb 2010 14:20:34 +0000
From: CHRISTIE GOWAN <christiegowan <@t> msn.com>
Subject: [Histonet] Fire in the lab
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT122-W196979242758728A9FC5EBAE3F0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Dear Histonet Friends,
I just wanted to share an incident we recently had with an old paraffin pot.
One of my techs came in on Sunday to embed some tissues, went into the
processor room and smelled something burning. He noticed our old paraffin
pot had charred looking labels on the outside so he went over, opened the
lid and poof!!! the pot went up in flames. The thermostat had gone haywire
and heated the paraffin to flash point. Opening the lid gave it the oxygen
it needed to ignite. He triggered the alarm, made the appropriate call and
then put it out with an extinguisher. Of course it kept re-igniting because
he could not get behind it to pull the plug. The fire dept finally was able
to get it pulled out and unplugged. Needless to say the tech was shaken and
the room was a mess. I applaud his courage and am not sure I would have done
the same. There was enough xylene and alcohol on the 4 processors to cause
quite an explosion but everything else was in a flammable cabinet. I was
wondering if thi
s type of thing had ever happened to anyone else?? Needless to say, we have
de-comissioned all old paraffin pots and will order only those with over
temp safety features. I guess I just wanted to remind everyone that fires
can happen in the lab and do probably more often than we hear about. This
was the first time for me and I have been in this business for over 20
years. Take care and be safe.
Christie Gowan HT (ASCP)
------------------------------
Message: 12
Date: Fri, 26 Feb 2010 09:50:08 -0500
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] Fire in the lab
To: CHRISTIE GOWAN <christiegowan <@t> msn.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF52397F58.C4D0BCA6-ON852576D6.00506B0D-852576D6.00517EC5 <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
Not exactly the same, but very similar. We had an automatic stainer by
the sink and one of the techs was washing glassware, the stainer was
running. The water apparently splashed on the wiring and a fire broke
out. We jumped into action. Just as we had been in-service. You are
correct what a mess to clean up! Fire extinquishers are wonderful but
extremely messy. We had totally taken care of the situation by the time
the fire department got here. We actually got accolades for preventing a
much larger fire. It was determined that there was some exposed wires on
the stainer.
A good lesson for all.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
CHRISTIE GOWAN <christiegowan <@t> msn.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/26/2010 09:21 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Fire in the lab
Dear Histonet Friends,
I just wanted to share an incident we recently had with an old paraffin
pot. One of my techs came in on Sunday to embed some tissues, went into
the processor room and smelled something burning. He noticed our old
paraffin pot had charred looking labels on the outside so he went over,
opened the lid and poof!!! the pot went up in flames. The thermostat had
gone haywire and heated the paraffin to flash point. Opening the lid gave
it the oxygen it needed to ignite. He triggered the alarm, made the
appropriate call and then put it out with an extinguisher. Of course it
kept re-igniting because he could not get behind it to pull the plug. The
fire dept finally was able to get it pulled out and unplugged. Needless to
say the tech was shaken and the room was a mess. I applaud his courage and
am not sure I would have done the same. There was enough xylene and
alcohol on the 4 processors to cause quite an explosion but everything
else was in a flammable cabinet. I was wondering if this type of thing had
ever happened to anyone else?? Needless to say, we have de-comissioned all
old paraffin pots and will order only those with over temp safety
features. I guess I just wanted to remind everyone that fires can happen
in the lab and do probably more often than we hear about. This was the
first time for me and I have been in this business for over 20 years. Take
care and be safe.
Christie Gowan HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 13
Date: Fri, 26 Feb 2010 16:36:30 +0100
From: Alexandra Meinl <alexandra.meinl <@t> gmail.com>
Subject: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC
slides
To: histonet <@t> lists.utsouthwestern.edu, histonet.nospam <@t> vneubert.com
Message-ID:
<b7eff7a1002260736x21396ddbhbab5fe6843b5cb7f <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello,
I'm glad that you already solved the problem your way.
I didn't read your first post, but we had exactly the same problem (and
we're also using cover plates). This artifact is very likely caused by tiny
air bubbles which are trapped under the cover plate. The crucial step is
when you drip a little buffer onto the plate in order to get your slide in
proper position. You get much lesser air bubbles if a) no detergent is used
and b) the PBS or TBS is at room temperature and not cold (which isn't good
anyway). We don't use detergents anymore (on coverplates).
Alexandra Meinl
************************************************
Dr. Alexandra Meinl
Ludwig Boltzmann Institute
for Experimental and Clinical Traumatology
Austrian Cluster for Tissue Regeneration
Histology
Donaueschingenstrasse 13
1200 Vienna - Austria
Contact @ Bernhard Gottlieb University School of Dentistry,
Waehringerstr. 25a, A-1090 Vienna
tel: +43 1 4277 67026
fax: +43 1 4277 67019
email: alexandra.meinl <@t> trauma.lbg.ac.at
------------------------------
Message: 14
Date: Fri, 26 Feb 2010 08:23:31 -0800
From: "Paula Lucas" <plucas <@t> biopath.org>
Subject: [Histonet] Saturday Tech Needed Orange County California
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201cab700$0987acd0$0f01a8c0 <@t> biopath.local>
Content-Type: text/plain; charset="us-ascii"
Hello histotechs- please email me if you're interested in working a few
hours on Saturday mornings. We are flexible, and have another Saturday tech
that can fill in if you can not work on certain dates. I'll give you more
info privately. I'm only seeking histotechs who have at least 2 years
experience embedding and cutting surgical/biopsy cases in a hospital or
private lab setting.
Thanks,
Paula
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
------------------------------
Message: 15
Date: Fri, 26 Feb 2010 11:03:25 -0600
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] ruo antibodies
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24A4826E8EF0964D86BC5317306F58A54257908393 <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
Our new CAP checklist does not mention the use of RUO antibodies anymore.
This was under the question using ASR antibodies in the past. I believe
the requirement was that if we wanted to use an RUO antibody we had to have
a disclaimer similar to the ASR disclaimer but we also had to have a
statement stating that we had searched for an IVD or ASR antibody. Does
anyone know if this is still the practice or am I missing something? I do
know of course than when using either an ASR or RUO antibody we have to
establish and verify the performance. Any thoughts about the RUO(s)?
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
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Message: 16
Date: Fri, 26 Feb 2010 11:57:50 -0600
From: Stephanie Rosenwinkel <rosensn <@t> live.com>
Subject: RE: [Histonet] Fire in the lab
To: <dkboyd <@t> chs.net>, <christiegowan <@t> msn.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <SNT113-W5234784F4DA482D8A9D80FDB3F0 <@t> phx.gbl>
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A few years back, I also had a fire break out while cleaning off the
weighing instrument. There was left over powders of other chemicals on there
and my gauze started on fire. So needless to say, please clean up after
yourselves! It was Scary, very scary! I tried to yell for help but nothing
would come out of my mouth, so I called 911.
It was a lesson learned the hard way, for sure.
Steph HT(ASCP)
> To: christiegowan <@t> msn.com
> From: DKBoyd <@t> chs.net
> Date: Fri, 26 Feb 2010 09:50:08 -0500
> Subject: Re: [Histonet] Fire in the lab
> CC: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
>
> Not exactly the same, but very similar. We had an automatic stainer by
> the sink and one of the techs was washing glassware, the stainer was
> running. The water apparently splashed on the wiring and a fire broke
> out. We jumped into action. Just as we had been in-service. You are
> correct what a mess to clean up! Fire extinquishers are wonderful but
> extremely messy. We had totally taken care of the situation by the time
> the fire department got here. We actually got accolades for preventing a
> much larger fire. It was determined that there was some exposed wires on
> the stainer.
> A good lesson for all.
>
> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
> Center I
> 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
> 804-765-5582 l dkboyd <@t> chs.net
>
>
>
>
>
>
>
> CHRISTIE GOWAN <christiegowan <@t> msn.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 02/26/2010 09:21 AM
>
> To
> <histonet <@t> lists.utsouthwestern.edu>
> cc
>
> Subject
> [Histonet] Fire in the lab
>
>
>
>
>
>
>
>
>
> Dear Histonet Friends,
>
> I just wanted to share an incident we recently had with an old paraffin
> pot. One of my techs came in on Sunday to embed some tissues, went into
> the processor room and smelled something burning. He noticed our old
> paraffin pot had charred looking labels on the outside so he went over,
> opened the lid and poof!!! the pot went up in flames. The thermostat had
> gone haywire and heated the paraffin to flash point. Opening the lid gave
> it the oxygen it needed to ignite. He triggered the alarm, made the
> appropriate call and then put it out with an extinguisher. Of course it
> kept re-igniting because he could not get behind it to pull the plug. The
> fire dept finally was able to get it pulled out and unplugged. Needless to
> say the tech was shaken and the room was a mess. I applaud his courage and
> am not sure I would have done the same. There was enough xylene and
> alcohol on the 4 processors to cause quite an explosion but everything
> else was in a flammable cabinet. I was wondering if this type of thing had
> ever happened to anyone else?? Needless to say, we have de-comissioned all
> old paraffin pots and will order only those with over temp safety
> features. I guess I just wanted to remind everyone that fires can happen
> in the lab and do probably more often than we hear about. This was the
> first time for me and I have been in this business for over 20 years. Take
> care and be safe.
>
> Christie Gowan HT (ASCP)
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