[Histonet] haematoxylin

[John Kiernan]

0.1G should give you an approximately half-oxidized hamatoxylin solution along the lines of Baker's haematal-8 or haematal-16, or Gill's haemalum. It should keep well. Precipitation must be due to something else you're doing. 
 
For the chemistry a good starting point is Conn's Biological Stains (10th ed, 2002). for information, see http://biologicalstaincommission.org and click on "Publications".
 
The current issue of Biotechnic & Histochemistry (Feb. 2010) is a special issue devoted to haematoxylin (Vol 85 No. 1). Last year the same journal (Vol 84 No. 4, pp. 159-177) carried an 18-page paper, "Nuclear staining with alum hematoxylin" by Bryan Llewellyn, which includes recipes for scores of haemalum mixtures and has much other interesting information. You can review the contents of the journal at http://informahealthcare.com/loi/bih. If your institution's library subscribes to the Informa Healthcare package of journals, you'll be able to download PDF files of articles - they go right back to 1925.
 
John Kiernan
Anatomy, UWO
London, Canada
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----- Original Message -----
From: Hana Peter <hana444 <@t> gmail.com>
Date: Thursday, February 25, 2010 17:16
Subject: [Histonet] haematoxylin
To: histonet <@t> lists.utsouthwestern.edu

> Hello everyone!
> Does anyone know where I can get more information about
> haematoxylin-haematein-oxyhaematein chemistry?
> 
> I have a problem with my Harris haematoxylin modification 
> (sodium iodate as oxidant). When I use a smaller amount of 
> sodium iodate (about 0,1g per 1g of haematoxylin) I have a huge 
> problem with precipitation. I don't know if this is because of 
> the unoxidized haematoxylin in staining solution or because of 
> something else.
> 
> Any help or suggestions are welcomed!
> Thanks in advance!
> Peter
> 
> 
> 
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