[Histonet] Snap Freezing Tissue

[Merced M Leiker]

In response to the "interested in other freezing techniques" part of 
Laurie's query:  We snap freeze by throwing a cyrovial of tissue (or a 
tissue piece wrapped in foil) into some liquid nitrogen for a couple 
minutes (vials will float, foil-wrapped pieces will sink). For controlled 
freezing of OCT-embedded tissues, I've frozen isopentane by pouring some in 
a liquid-nitrogen proof shallow container (such as a pipet-tip box lid) and 
floating it on the liquid nitrogen til frozen. I guess this works if you're 
processing a relatively small number of samples at a time, which, if you 
work in a hospital, sounds like you may not be.  Additionally, we've made 
alcohol-dry ice slurries in styrofoam containers in which to freeze 
OCT-embedded tissues.

Regards,
Merced

--On Thursday, February 25, 2010 10:12 AM -0500 Michael W
illiams <mjwilliams <@t> scilogex.com> wrote:

> It can be purchased directly from 3M. You may also find some local
> suppliers.
>
> Michael Williams
> mjwilliams <@t> scilogex.com
> SCILOGEX, LLC
> TF: (877) SCILOGEX
> T: (860) 828-5614
> F: (860) 828-5389
> www.SCILOGEX.com
>
>
> -----Original Message-----
> From: Houston, Ronald [mailto:Ronald.Houston <@t> nationwidechildrens.org]
> Sent: Thursday, February 25, 2010 9:42 AM
> To: Michael Williams; Laurie Colbert
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Snap Freezing Tissue
>
> Who supplies the 3MT Novec Engineered Fluid HFE-7100?
> Thanks
>
> Ronnie Houston
> Anatomic Pathology Manager
> Nationwide Children's Hospital
> Columbus OH 43205
> (614) 722 5450
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
> Williams
> Sent: Thursday, February 25, 2010 8:55 AM
> To: 'Laurie Colbert'
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Snap Freezing Tissue
>
> Hi Laurie,
>
> We distribute Bright's Clini-RF. We hear good reports about using 3MT
> NovecT
> Engineered Fluid HFE-7100 which is inert and has an excellent
> environmental,
> health and safety profile.
>
> Regards,
>
> Michael Williams
> mjwilliams <@t> scilogex.com
> SCILOGEX, LLC
> TF: (877) SCILOGEX
> T: (860) 828-5614
> F: (860) 828-5389
> www.SCILOGEX.com
>
>
> -----Original Message-----
> From: Laurie Colbert [mailto:laurie.colbert <@t> huntingtonhospital.com]
> Sent: Wednesday, February 24, 2010 4:55 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Steve Pike
> Subject: [Histonet] Snap Freezing Tissue
>
> I was wondering how others are snap freezing tissue?  We have been using
> a histobath (a refrigerator device that cools isopentane for freezing
> tissue), but it has died.  I would love to have another Histobath, but
> they are no longer carried by the vendor that I originally bought mine
> from.  Does anyone know where I can get either a new or refurbished
> Histobath (vendor calls are welcome)?  I'm also interested in other
> freezing techniques.
>
>
>
> Laurie Colbert
>
> Huntington Hospital
>
> Pasadena, CA
>
> (626) 397-8620
>
>
>
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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