[Histonet] Re: [ Histonet ] Tris Buffer Preservative

[histonet.nospam <@t> vneubert.com]

I used to sterilze my TBS by autoclaving it.
For aliquots  I used TBS with 0,1% sodium acide.
I never had any problems with bacteria; my 10x TBS concentrate was fine for weeks.

----- Original Message -----
From: amosbrooks <@t> gmail.com
To: histonet <@t> lists.utsouthwestern.edu
Date: 24.02.2010 17:59:45
Subject: [Histonet] Tris Buffer Preservative


> Hi,
>    I have been making up my own tris buffer for immunohistochemistry. It has
> been working swimmingly, with one exception. I have been doing some work on
> Salmonella, and apparently the researcher has been seeing bacteria in her
> immunofluorescent slides that she says are moving and seem to be viable. She
> wanted to culture all my reagents. So the primary and secondary antibody
> diluents (commercially purchased) came up clean, but the TBS ended up with a
> pretty healthy growing colony. Now since I don't do IHC overnight in an
> incubator, I don't think this is necessarily a catastrophy. (No one else has
> noticed this) It does seem to warrant further investigation though. So for
> the folks that make their own solutions up, what do you use as a
> preservative for your buffers and how much do you use. I haven't seen
> anything in any of the recipes I have found. I was thinking Sodium Azide,
> but it is really hazardous, and Wikipedia says it is actually explosive (
> http://en.wikipedia.org/wiki/Sodium_azide). Biocare Medical's data sheet
> says they use less than 0.25% procyclin. (thanks for not hiding the
> ingredients Biocare, I love you for that!) Has anyone tried that? Any other
> suggestions would be welcome.
> 
> Thanks,
> Amos
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