[Histonet] Sirius Red

[Nails, Felton]

Thanks for everyone's help

________________________________
From: John Kiernan [mailto:jkiernan <@t> uwo.ca]
Sent: Wednesday, February 24, 2010 11:36 AM
To: Nails, Felton
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Sirius Red

You'll find brief instructions for the picro-sirius red method for collagen in nearly every histological techniques book published since about 1970. A Google search for "sirius red vendor" brings up 484 hits, though not all are for vendors. It's important to get the correct dye (C.I. 35780, Direct red 80). For a thorough discussion of how to use the method intelligently, see L. Junqueira et al 1979. Histochem. J. 11: 447-455.
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Date: Wednesday, February 24, 2010 12:23
Subject: [Histonet] Sirius Red
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

>  Is there a good Sirius Red procedure used to stain collagen
> And where can I find the powder
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