Re: Re: [Histonet] Double labeling with antibodies that need differentfixatives

[TF]

what is the other marker than CD31?
May you let us know?


2010-02-23 



TF 



发件人： Adam . 
发送时间： 2010-02-23  10:55:18 
收件人： Phebe Verbrugghe 
抄送： histonet 
主题： Re: [Histonet] Double labeling with antibodies that need differentfixatives 
 
Although I haven't tried it myself, others have gotten CD31 from BD to work
on FFPE tissue using the tyramide amplification system on zinc buffered
formalin fixed sections. I've generally had good luck with zinc buffered
formalin myself for many antigens so it may work for your other one.
See http://www.ncbi.nlm.nih.gov/pubmed/19052548
Just to be clear, they used zinc buffered formalin, which isn't the same
thing as zinc fixative.
Adam
On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe <
pverbrugghe <@t> meddent.uwa.edu.au> wrote:
>
> Hi all,
>
> I would like to do an immunofluorescent double labeling with two
> antibodies but 1 antibody works on acetone fixed frozen tissue but not
> on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370)
> and the other one works on formalin fixed paraffin embedded tissue but
> not on acetone fixed frozen tissue. Is there any way I could still do a
> double labeling and how?
> Also, does anyone have experience with zinc fixative? If my antibody
> works on formalin fixed tissue is it likely to also work on zinc fixed
> tissue?
>
> Thank you very much in advance,
>
> Phebe
>
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