[Histonet] Bone tissue

[Rene J Buesa]

First of all, Mollifex or any other alkaline substance will do nothing "useful" to the bone.
I tend to think that you processed the bone before it was completely decalcified and that is the cause for an incomplete infiltration and a subsequent difficult sectioning.
René J.

--- On Mon, 2/22/10, Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org> wrote:


From: Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org>
Subject: [Histonet] Bone tissue
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, February 22, 2010, 4:36 PM


We have a difficulty cutting metatarsal bone . It seems that our sections are so dried up. I was thinking that our dehydration have something to do with this which we have placed it in a wrong processing procedure for our large bone. The tissue is 4 mm thick and 1-2 cm in length and width and  was dehydrated in 70% - 4 hrs, 80%-4 hrs,95% -4 hrs and 2 changes of 100% 3 hrs each, paraffin is 4 hrs each 2 changes. The tissue was decalcified in 14% EDTA. When we start cutting them it is so brittle and we could not even create a section. I have surfaced decal it and also place in a softener Mollifex some of it work but some does not work. Please help us remedy this tissue. Thank you.




Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768



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