[Histonet] EDTA

[Andrea T. Hooper]

Although what you say is true, it is worth noting that there are some antigens/antibodies more amenable to acid decal. I have experienced this myself several times recently and been burned by thinking EDTA will always give better IHC results. At least for murine bone marrow vasculature antigens, this appears to be the case for some antibodies.  Unfortunately as with most things in histology, there is always exceptions to rules of thumb!

--- On Fri, 2/19/10, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:


From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] EDTA
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>, "Dorothy LWebb" <Dorothy.L.Webb <@t> HealthPartners.Com>
Date: Friday, February 19, 2010, 9:03 PM


EDTA (which stands for Ethylene-diamino-tratacetic-acid) is a chelating agent and the method of choice to decalcify BM biopsies.
The thing is that it should be used alone, and not combined with any acid, as you state (not even formic acid).
By itself will produce an extremely gentle decalcification that will be completely suitable for IHC studies.
René J.


--- On Fri, 2/19/10, Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:


From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] EDTA
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, February 19, 2010, 1:29 PM


I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix.  I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens.

Thank you ahead of time for your advice!

Dorothy Webb, HT (ASCP)
Regions Histology Technical Supervisor
651-254-2962



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