[Histonet] frozen sections of cartilage

[Rene J Buesa]

You should aim at obtaining 4 objectives:
1- extremely thin sections (as thin as you are able to produce);
2- absolutely wrinkle free;
3- using positivey charged slides, and
4- let the sections air dry completely before starting the IHC
René J.

--- On Mon, 2/22/10, Kim Merriam <kmerriam2003 <@t> yahoo.com> wrote:


From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] frozen sections of cartilage
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, February 22, 2010, 2:37 PM


Hi Everyone,

Any tips for keeping frozen sections of cartilage from falling off the slides during IHC staining?  We should not have to do HIER, but they still fall of the slides quite easily.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



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