[Histonet] IF staining on peritoneal macrophages

[Adam .]

When I did it, I think I used our standard sort buffer, which is 0.2% BSA in
PBS pH 7.4. I've also read that you can use anything with serum, typically
5-10%. The key seems to be prewetting the membranes with something with
protein.

I usually cytospin them and briefly (30 seconds) air dry them until there is
no obvious liquid, before I added the fixative. I managed to get usable (but
not ideal) cytospins from as few as 1000 cells.

Let me know if it works,
Adam

On Fri, Feb 19, 2010 at 9:47 AM, Mauricio Avigdor
<bitesizellama <@t> gmail.com>wrote:

> Thank you Adam and Jay for your replies.
>
> Cytoslides are the pre-marked slides for use with the Cytospin -
> http://www.thermo.com/com/cda/product/detail/0,1055,21035,00.html
>
> Adam, do you have a recommended concentration of BSA for this? Also, do you
> air dry the slides prior to fixation? Do you air dry afterwards? Thanks
> again for your help.
>
> Mauricio
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