[Histonet] IHC Question
Leiferman, Ellen
Leiferman <@t> ortho.wisc.edu
Wed Feb 17 11:29:11 CST 2010
Hi All
I’m interested in performing IHC on a mouse ligament. Given their minuscule size, in lieu of paraffin or cryostat embedding and sectioning, we would like to embed in plastic and section at 1 micrometer. Things we are interested in detecting include collagen type 1 and III, CD31, Arginase 1 and iNOS---to name a few. Is there a plastic out there that is recommended for Immuno work? I have performed a brief literature search and have come across Immunobed, JV-4 as well as LR White and Lowicryl. Is anyone familiar with any of these and whether I would have any success? I am familiar with Epon-Araldite embedding and sectioning with glass knives, but have never used any of the above plastics.
Thanks for any info
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