[Histonet] Stain for Smooth Muscle Cells

Jan Shivers shive003 <@t> umn.edu
Wed Feb 17 09:10:10 CST 2010


Smooth Muscle Actin; Dako; cat. # M0851, clone 1A4

Requires HIER antigen retrieval (I use a Biocare Decloaking Chamber with 
Dako Target Retrieval Solution)
Strong staining on sheep tissue

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 17, 2010 8:49 AM
Subject: Re: [Histonet] Stain for Smooth Muscle Cells


Hi all,

What stain do most people use to demonstrate smooth muscle cells?  I know 
there
are various choices....We are looking at sheep lung.

Thanks!

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org




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