[Histonet] SEM for Spermatozoa
Jamshid Amiri Moghaddam
j_amiri <@t> modares.ac.ir
Tue Feb 16 07:03:34 CST 2010
Hi every body
We want to scan fish spermatozoa By SEM. But I don't know the method for
dehydration after Post fixation with osmium tetra oxide? By ethanol series
or aceton?
I thanks to all who can help me in SEM for Spermatozoa.
Regards: Jamshid
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
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Sent: None
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 75, Issue 20
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Today's Topics:
1. Hi to all............. (Don Hammer)
2. IHC TBS wash buffer (Justin Peters)
3. Florida Society for Histotechnology-Region III Meeting
(Jerry Santiago)
4. QIHC (Walter Benton)
5. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar)
6. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar)
7. Re: IHC TBS wash buffer (V. Neubert)
8. November 2009 copy of ASCP Lab Medicine journal (Justin Peters)
9. Re: November 2009 copy of ASCP Lab Medicine journal (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Sun, 14 Feb 2010 22:02:27 -0800
From: "Don Hammer" <donh7 <@t> earthlink.net>
Subject: [Histonet] Hi to all.............
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <281414D805204848BA1CC3D07AB02D96 <@t> Don>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histology Professionals,
I just finished reading the recent issue of "NSH In Action", noting a
reference to "Histonet" so thought to drop in to see how this very
important, long lasting exchange of information is going.
Congrats Linda with thanks to the UTSouthwestern for supporting the site.
(14 years now I believe)
Perhaps there are a few colleagues still here, continuing to offer
assistance from their wealth of experience and knowledge, from back in my
Laboratory days (or was that Daze?) :>) It's been near 12 years since
retiring, wouldn't give it up for anything, but often think of you all who
are in the Profession and now preparing for "Histotechnology Professionals
Day" Such a great opportunity to blow your own horn a bit while making the
general public aware of your support in the various facets of Medicine.
Don't forget to celebrate all you do on a daily basis.
I'm completely blown away at how NSH has grown from our Founding Meeting 36
years ago with it's extensive Symposium/Convention, Educational Materials,
Journal of Histotechnology, Summer Symposium's, Teleconferences, Specialty
Forums, Awards, Positions with or on Boards of National Organizations, just
to highlight a few. When viewing the Website containing so much info for
Members it's hard to believe much of it was kept on Card files and File
drawers manned by one person in the Office. What's difficult to understand
is the total Membership is nearly the same number.
Well just sending some thoughts. Think I figured out how to post a comment,
now I need to go figure out how to view the daily posts on "Histonet" It's
been so long since doing that.
Don Hammer, Retired Guy
" I can't remember the last time I wasn't at least kind of tired."
"I totally take back all those times I didn't want to nap when I
was younger"
------------------------------
Message: 2
Date: Mon, 15 Feb 2010 08:24:41 -0500
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] IHC TBS wash buffer
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24D22DE9E488AA43BF92A4389F2DDB1F0ECA0BC3 <@t> mail1.BOSTWICK.COM>
Content-Type: text/plain; charset="us-ascii"
Can anyone tell me what an acceptable pH range for IHC TBS wash buffer
is?
------------------------------
Message: 3
Date: Mon, 15 Feb 2010 05:29:08 -0800 (PST)
From: Jerry Santiago <jsantiago <@t> bellsouth.net>
Subject: [Histonet] Florida Society for Histotechnology-Region III
Meeting
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <967631.97107.qm <@t> web180415.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Dear Histonetters,
The program and online registration for the Florida Society for
Histotechnology Meeting is now available at www.fshgroup.org. You can also
go directly to the online registration process by visiting
www.fshmeeting.com.
Sincerely,
Jerry Santiago, BS, HTL(ASCP)QIHC
NSH Region III Director
Shands Jacksonville
T: 904-244-6149
E-mail: santij1 <@t> bellsouth.net
------------------------------
Message: 4
Date: Mon, 15 Feb 2010 09:13:14 -0500
From: "Walter Benton" <WBENTON <@t> umm.edu>
Subject: [Histonet] QIHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4B790FC5.D886.00F4.3 <@t> umm.edu>
Content-Type: text/plain; charset=US-ASCII
I recently took and passed the examination . I used the Dako book, material
from Ethel Macrea's presentation, NSH study guide and material from the
other references on the list.
Ethel can be reached at emacrea <@t> swskinpath.com
She is a wonderful resource.
Message: 6
Date: Fri, 12 Feb 2010 20:22:01 -0500
From: jfi786 <@t> aol.com
Subject: [Histonet] QIHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CC7A6213F16820-47FC-8816 <@t> webmail-m018.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Hello, I was wondering if anyone out there had any tips on passing the QIHC,
I have studied with the Dako book but unfortunately I did not pass at my
first attempt. If there are any sample questions or packages available I
would really like to get them.
Thanks
fj
Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508
This e-mail and any accompanying attachments may be privileged,
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------------------------------
Message: 5
Date: Mon, 15 Feb 2010 09:24:26 -0500
From: "Fawn Bomar" <Fawn.Bomar <@t> HalifaxRegional.com>
Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E779667A9118F74D8B7D82A1891BE0300274FCDD30 <@t> exch-2k7.hrhs.com>
Content-Type: text/plain; charset=us-ascii
Hi everyone,
Since I received this e-mail from Lynne about a question that I asked, I
decided it was probably in my best interest not to ask any more questions in
general. Thank you to everyone that helped verify that my validation
process was indeed up to par! I will stay on the histonet in hopes that I
can help others but I will keep my own questions to myself
Fawn
________________________________________
From: Jones, Lynne [jonesly <@t> mir.wustl.edu]
Sent: Friday, February 12, 2010 5:48 PM
To: Fawn Bomar
Subject: FYI - Histonet Digest, Vol 75, Issue 17
Hello -
I was curious about the facility where you worked, so I looked up Halifax
Regional, but it seems congratulations are in order, since Gwen Wade just
posted a Press release indicating that the lab passed CAP accreditation.
(Though I'm not sure your inquiry about a fundamental process in a histology
lab would inspire confidence.)
HistoNet is a fabulous resource, but there are some questions you may want
to ask using a less traceable e-mail account. HistoNet archives are readily
accessible, even to the general public. I do understand the panic and
second-guessing that hits right before the inspectors appear, but you
obviously had all the I's dotted and t's crossed. The next time will be
easier. :)
Take care,
Lynne
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-
------------------------------
Message: 3
Date: Thu, 11 Feb 2010 14:06:56 -0500
From: "Fawn Bomar" <Fawn.Bomar <@t> HalifaxRegional.com>
Subject: [Histonet] Help...
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E779667A9118F74D8B7D82A1891BE0300274FCDD28 <@t> exch-2k7.hrhs.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone have a protocol or procedure for validating the Her2 antibody?
We are trying to get ready for a CAP inspection and I'm not sure if what we
did is ok or not.
Thank you in advance
Fawn
-------------------------------------------------------------
This electronic message may contain information that is
confidential or legally privileged. It is intended only
for the use of the individual(s) and entity named as recipients
in the message.
If you are not an intended recipient of this message, please
notify the sender immediately and delete the material from any
computer. Do not deliver, distribute, or copy this message, and
do not disclose its contents or take any action in reliance on
the information it contains.
Thank you
------------------------------
Message: 4
Date: Thu, 11 Feb 2010 12:28:27 -0700
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Help...
To: "Fawn Bomar" <Fawn.Bomar <@t> HalifaxRegional.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B100C41 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
Fawn
CAP has specific quidelines, its right there in the questions, did you
do what that question is asking and have you documented what was done.
They tell you right in the check sheet what needs to be completed. Test
validation must be performed on a minimum of 25 cases (they recommend
25- 100) and they tell you how to validate too. There is also an
article that you can get on the CAP website on IHC standardization. I
have a pdf of it here if you need it.
LIz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fawn
Bomar
Sent: Thursday, February 11, 2010 12:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Help...
Does anyone have a protocol or procedure for validating the Her2
antibody? We are trying to get ready for a CAP inspection and I'm not
sure if what we did is ok or not.
Thank you in advance
Fawn
-------------------------------------------------------------
This electronic message may contain information that is
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If you are not an intended recipient of this message, please
notify the sender immediately and delete the material from any
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do not disclose its contents or take any action in reliance on
the information it contains.
Thank you
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 75, Issue 17
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Message: 6
Date: Mon, 15 Feb 2010 09:29:45 -0500
From: "Fawn Bomar" <Fawn.Bomar <@t> HalifaxRegional.com>
Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E779667A9118F74D8B7D82A1891BE0300274FCDD31 <@t> exch-2k7.hrhs.com>
Content-Type: text/plain; charset=us-ascii
One more thing.... This was my reply to Lynn and her response to my
question.
Fawn
________________________________________
From: Fawn Bomar
Sent: Monday, February 15, 2010 9:24 AM
To: Jones, Lynne
Subject: RE: FYI - Histonet Digest, Vol 75, Issue 17
Hi Lynne,
My inquiry about the Her2 validation was to get overall suggestions on how
different labs interpreted how to go about the validation process. I said
that we had a process but since I was new to the clinical aspect and to
immuno's in general, I wanted to make sure that we were up to par with
everyone else or if I needed to make adjustments to make our validation
better. I was making sure that we met or exceeded expectations and I felt
that everyone could offer an opinion. There should be no doubt in
confidence in our accreditation...there should be some comfort in knowing
that I am making sure that we are doing the best work that can be done.
Sincerely
Fawn
________________________________________
From: Jones, Lynne [jonesly <@t> mir.wustl.edu]
Sent: Friday, February 12, 2010 5:48 PM
To: Fawn Bomar
Subject: FYI - Histonet Digest, Vol 75, Issue 17
Hello -
I was curious about the facility where you worked, so I looked up Halifax
Regional, but it seems congratulations are in order, since Gwen Wade just
posted a Press release indicating that the lab passed CAP accreditation.
(Though I'm not sure your inquiry about a fundamental process in a histology
lab would inspire confidence.)
HistoNet is a fabulous resource, but there are some questions you may want
to ask using a less traceable e-mail account. HistoNet archives are readily
accessible, even to the general public. I do understand the panic and
second-guessing that hits right before the inspectors appear, but you
obviously had all the I's dotted and t's crossed. The next time will be
easier. :)
Take care,
Lynne
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-
------------------------------
Message: 3
Date: Thu, 11 Feb 2010 14:06:56 -0500
From: "Fawn Bomar" <Fawn.Bomar <@t> HalifaxRegional.com>
Subject: [Histonet] Help...
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E779667A9118F74D8B7D82A1891BE0300274FCDD28 <@t> exch-2k7.hrhs.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone have a protocol or procedure for validating the Her2 antibody?
We are trying to get ready for a CAP inspection and I'm not sure if what we
did is ok or not.
Thank you in advance
Fawn
-------------------------------------------------------------
This electronic message may contain information that is
confidential or legally privileged. It is intended only
for the use of the individual(s) and entity named as recipients
in the message.
If you are not an intended recipient of this message, please
notify the sender immediately and delete the material from any
computer. Do not deliver, distribute, or copy this message, and
do not disclose its contents or take any action in reliance on
the information it contains.
Thank you
------------------------------
Message: 4
Date: Thu, 11 Feb 2010 12:28:27 -0700
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Help...
To: "Fawn Bomar" <Fawn.Bomar <@t> HalifaxRegional.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B100C41 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
Fawn
CAP has specific quidelines, its right there in the questions, did you
do what that question is asking and have you documented what was done.
They tell you right in the check sheet what needs to be completed. Test
validation must be performed on a minimum of 25 cases (they recommend
25- 100) and they tell you how to validate too. There is also an
article that you can get on the CAP website on IHC standardization. I
have a pdf of it here if you need it.
LIz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fawn
Bomar
Sent: Thursday, February 11, 2010 12:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Help...
Does anyone have a protocol or procedure for validating the Her2
antibody? We are trying to get ready for a CAP inspection and I'm not
sure if what we did is ok or not.
Thank you in advance
Fawn
-------------------------------------------------------------
This electronic message may contain information that is
confidential or legally privileged. It is intended only
for the use of the individual(s) and entity named as recipients
in the message.
If you are not an intended recipient of this message, please
notify the sender immediately and delete the material from any
computer. Do not deliver, distribute, or copy this message, and
do not disclose its contents or take any action in reliance on
the information it contains.
Thank you
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
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End of Histonet Digest, Vol 75, Issue 17
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The material in this message is private and may contain Protected Healthcare
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Message: 7
Date: Mon, 15 Feb 2010 15:55:00 +0100
From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
Subject: Re: [Histonet] IHC TBS wash buffer
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4B796044.9080301 <@t> vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1
7,6
;)
> Can anyone tell me what an acceptable pH range for IHC TBS wash buffer
> is?
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 8
Date: Mon, 15 Feb 2010 10:45:35 -0500
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24D22DE9E488AA43BF92A4389F2DDB1F0ECA0E04 <@t> mail1.BOSTWICK.COM>
Content-Type: text/plain; charset="us-ascii"
Does anyone have a copy of the November 2009 ASCP Lab Medicine journal
that they wouldn't mind parting with? I have a colleague who's wife had
a paper published in that edition and he would like to have a copy.
Thanks!
Justin Peters, HTL (ASCP)
4355 Innslake Drive
Glen Allen, Virginia 23060
Phone: (804) 967-9225 ext. 1831
Cell: (804) 822-6084
------------------------------
Message: 9
Date: Mon, 15 Feb 2010 07:50:18 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] November 2009 copy of ASCP Lab Medicine
journal
To: histonet <@t> lists.utsouthwestern.edu, Justin Peters
<JPeters <@t> bostwicklaboratories.com>
Message-ID: <288692.27975.qm <@t> web65708.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Just out of curiosity: isn't your colleague's wife entitled to a copy of the
issue where she published the article?
Reni J.
--- On Mon, 2/15/10, Justin Peters <JPeters <@t> bostwicklaboratories.com> wrote:
From: Justin Peters <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, February 15, 2010, 10:45 AM
Does anyone have a copy of the November 2009 ASCP Lab Medicine journal
that they wouldn't mind parting with? I have a colleague who's wife had
a paper published in that edition and he would like to have a copy.
Thanks!
Justin Peters, HTL (ASCP)
4355 Innslake Drive
Glen Allen, Virginia 23060
Phone: (804) 967-9225 ext. 1831
Cell: (804) 822-6084
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 75, Issue 20
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