[Histonet] Re: Alternatives to BioQuant for Bone Histomorphometry

[Nathanael Reveal]

Hi Adam,

Sorry the BIOQUANT is causing so much frustration for you!

It certainly sounds like the protocol that was suggested to you isn't best
tool for your work. Could I ask you to send me an image of the tissue you
need to analyze? The thresholding tools tend to work best with
non-demineralized tissue stained with one of the trichrome stains. You're
right the thresholding isn't great with H&E on TRAP.

There are some fairly straightforward manual tracing protocols that might do
better as in: choose the Bone Surface array and trace it on the image.
Choose the Oc.N array and click the osteoclasts in the image.

To help document a more relevant protocol, we can put together a custom
training video for you kind of like the one here:

http://download.bioquant.com/digital-pathology

I'd be glad to handle the support details off the list if you like.

Best,
Nathanael


---
Nathanael Reveal, President
BIOQUANT Image Analysis Corporation

5611 Ohio Avenue, Nashville, TN 37209
phone: 615-350-7866, toll free: 800-221-0549, fax: 615-350-7282

email: nathanael <@t> bioquant.com, web: www.bioquant.com

My life is an indivisible whole, and all my activities run into one another
and they have their rise in my insatiable love of humanity.
Mohandas K. Gandhi

--QUOTE--
Message: 12
Date: Fri, 5 Feb 2010 16:38:05 -0600
From: "Adam ." <anonwums1 <@t> gmail.com>
Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
       <858249121002051438h85948f4h896f6676fad94f3d <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi all,

I am looking for an alternative program to BioQuant for bone
histomorphometry. We need to quantify the number of osteoblasts /
osteoclasts per bone surface area as well as the percent surface area
occupied by those cells. We have a computer with BioQuant on it available,
but we find the software to be incredibly clunky and often nearly impossible
to use. Based on my limited attempts to use it, it very well might rank as
one of the worst user interfaces I've ever seen, and I was trained in
computer science and have seen my fair share of horrible software (I'm
looking at you, Lotus Notes).

Anyhow, any suggestions on a (preferably cheap / free) replacement for doing
simple analysis or how to make BioQuant less painful would be very helpful.

Thanks,
Adam