[Histonet] Slide baking before IHC

[Morken, Tim]

Much of the information on slide drying has to be gleaned from books and is mostly the personal experience of various authors.

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net]
Sent: Sunday, February 07, 2010 7:55 AM
To: Morken, Tim; 'Pat Laurie'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide baking before IHC

I concur with Tim, this paper is a good reference for slide drying temps, as
with everything else there is no rule that works for every antigen, I play
it safe and try very hard to air dry draining until there is no water left
on or under the section, and then I bake at temps not above 60dc for most
things, but when it comes to bone and cartilage, I drain and air dry them
and then lay them flat on heat plate at 45 dc overnight when possible.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Tim
Sent: Friday, February 05, 2010 10:24 AM
To: Pat Laurie; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide baking before IHC

Here is the reference:

Effect of Slide Drying at 80Deg C on Immunohistochemistry
A.F. Henwood
J Histotechnology, VOl. 28, no. 1, March 2005

If you are an NSH member you can email the NSH office and ask for a pdf
reprint.

The author compares heating the slides at 80C for seven hours to one hour at
65C. Some antigens were adversely affected, some were not.



Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat Laurie
Sent: Friday, February 05, 2010 12:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide baking before IHC

Histonet,

I have heard rather anecdotally that if you bake slides at high temps (75
degrees to 80 degrees) before IHC for a long period of time (several hours
to days), you may affect antigenicity for some antibodies.  Has there been
any study done about this?  Also, what if it is a high temp (around 75
degrees C) for just 20 minutes?  Or a low temperature for a long period of
time?

Thanks in advance for your input.

--
Patrick Laurie HT(ASCP)QIHC
plaurie <@t> cellnetix.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet