[Histonet] Alternatives to BioQuant for Bone Histomorphometry

[Adam .]

I am planning on using IHC using DAB stain to label the cells of interest (a
subpopulation of bone cells) counterstained with hematoxylin. My coworker
wants to do similar things with TRAP stained osteoclasts. The way I was
trained how to do this would be turn off thresholding completely (the guy
said it doesn't work well enough), outline the contours of the bone, and
then outline the perimeter lined by the cells of interest, and finally click
on each cell of interest.

It seemed to me that doing this in BioQuant was needlessly complex and
involved constantly loading and resetting these vectors each time, and if
you forgot to do that, you could do a whole bunch of work and have BioQuant
either compute the parameters completely wrong or simply discard what you
did.

Adam

On Mon, Feb 8, 2010 at 6:45 AM, Jack Ratliff <ratliffjack <@t> hotmail.com>wrote:

> Adam,
>
> What stain are you using for your quantitation and are you trying to
> perform measurements via the "thresholding" feature?
>
>
> Jack
>
>
> On Feb 5, 2010, at 4:38 PM, "Adam ." <anonwums1 <@t> gmail.com> wrote:
>
>  Hi all,
>>
>> I am looking for an alternative program to BioQuant for bone
>> histomorphometry. We need to quantify the number of osteoblasts /
>> osteoclasts per bone surface area as well as the percent surface area
>> occupied by those cells. We have a computer with BioQuant on it available,
>> but we find the software to be incredibly clunky and often nearly
>> impossible
>> to use. Based on my limited attempts to use it, it very well might rank as
>> one of the worst user interfaces I've ever seen, and I was trained in
>> computer science and have seen my fair share of horrible software (I'm
>> looking at you, Lotus Notes).
>>
>> Anyhow, any suggestions on a (preferably cheap / free) replacement for
>> doing
>> simple analysis or how to make BioQuant less painful would be very
>> helpful.
>>
>> Thanks,
>> Adam
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>>