[Histonet] xylene alternatives

[Finley, Sue [PH]]

Why not look at processing that excludes the use of xylene all together  ie milestone pathos tissue processor.  

I am not a sales rep only a very happy end user

Regards
sue

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 04, 2010 12:45 PM
To: KelleyDurden; Jay Lundgren
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] xylene alternatives

There is no better alternative than not using xylene or any of its "so called" substitutes to get the best infiltration possible with the lowest infiltration gradient possible.
René J.

--- On Thu, 2/4/10, Jay Lundgren <jaylundgren <@t> gmail.com> wrote:


From: Jay Lundgren <jaylundgren <@t> gmail.com>
Subject: Re: [Histonet] xylene alternatives
To: "Durden, Kelley" <kelleydurden <@t> pathology.ufl.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, February 4, 2010, 12:53 PM


When you add in the cost of disposing of your xylene, or the cost of
distilling it (including tech time), xylene substitutes come closer to the
cost of xylene.

                                                Jay A. Lundgren M.S., HTL
(ASCP)

On Thu, Feb 4, 2010 at 12:45 PM, Durden, Kelley <
kelleydurden <@t> pathology.ufl.edu> wrote:

> Nothing works as well as xylene.  We have tried several different
> substitutes and nothing has provided consistent results.  Xylene has a
> slight tolerance for water.  Unlike xylene substitutes.  If any water is
> present in the substitute before coverslipping the results are disastrous.
>  So unless you have a very small volume or change your pre xylene alcohols
> several times a day you may end up with sub standard results.  Plus
> substitutes are more expensive - generally.
>
> Kelley
>
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