[Histonet] xylene alternatives

[Rathborne, Toni]

Why? They should both be disposed of in the same way.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jay
Lundgren
Sent: Thursday, February 04, 2010 12:54 PM
To: Durden, Kelley
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] xylene alternatives


When you add in the cost of disposing of your xylene, or the cost of
distilling it (including tech time), xylene substitutes come closer to the
cost of xylene.

                                                Jay A. Lundgren M.S., HTL
(ASCP)

On Thu, Feb 4, 2010 at 12:45 PM, Durden, Kelley <
kelleydurden <@t> pathology.ufl.edu> wrote:

> Nothing works as well as xylene.  We have tried several different
> substitutes and nothing has provided consistent results.  Xylene has a
> slight tolerance for water.  Unlike xylene substitutes.  If any water is
> present in the substitute before coverslipping the results are disastrous.
>  So unless you have a very small volume or change your pre xylene alcohols
> several times a day you may end up with sub standard results.  Plus
> substitutes are more expensive - generally.
>
> Kelley
>
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