[Histonet] xylene alternatives

[Durden, Kelley]

Nothing works as well as xylene.  We have tried several different substitutes and nothing has provided consistent results.  Xylene has a slight tolerance for water.  Unlike xylene substitutes.  If any water is present in the substitute before coverslipping the results are disastrous.  So unless you have a very small volume or change your pre xylene alcohols several times a day you may end up with sub standard results.  Plus substitutes are more expensive - generally.

Kelley