[Histonet] Eosin leaching out of sections

[Joyce Cline]

Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain.

The beads are ordered from Amercian Mastertech.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
Hendricksen
Sent: 03 February 2010 04:16
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections

Hi again,

Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an H&E stained slide?

Thanks,

Scott Hendricksen HT(ASCP)
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