[Histonet] Alcohol fixation and immuno

[Jay Lundgren]

    You did not specify which enzyme you were using, but I am assuming
proteinase K.  If an hour's retrieval is too much, and no retrieval is too
little, I would suggest running some (alcohol fixed) controls at 15, 30, and
45 minutes in the 95C, or 1,2,3, and 4 minutes in the enzyme.  Evaluate the
slides with a pathologist for staining vs. section adhesion.

                                                    Jay A. Lundgren M.S.,
HTL (ASCP)

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