[Histonet] Tips for drying slides before H&E stain.

[Tony Henwood]

I would suggest 65oC for 20-25 minutes.
High temperature drying will affect IPX results and, especially with
under-fixed tissues, adversely affect H&E staining.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
Hendricksen
Sent: Wednesday, 3 February 2010 3:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tips for drying slides before H&E stain.


Hi,
	
Our lab just received a slide drying oven, and we are trying to figure
out what's a good temperature for the slides to be heated and for how
long. We are mainly doing H&E slides. 

The way we are currently drying them is under a small fan, then heat
them up, and back to the fan. Then finally heating them up and off to
the stainer.

Just putting them in the slide drying oven for 10 min at 80C melts the
paraffin, but leaves some water on the bottom of some sections.

Any suggestions to get the slides dry asap ?  Also is the oven much help
to your labs?

Thanks !

Scott Hendricksen HT(ASCP)
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