[Histonet] M-1 embedding matrix question

[Kwan, Joyce]

Hello Histonetters,

 

I am using Shandon M-1 embedding matrix for cryosectioning perfused,
cryo-protected spinal cord.  The embedding media allows me to section
without the curling you frequently get with OCT compound.  One thing I
am noticing, however, is the M-1 media does not dissolve very well, even
in several washes in water/PBS, and dehydrations in ethanol and xylene.
Consequently, the M-1 residue stains along with the histological stain.
OCT dissolves cleanly.   Have any of you experienced this, or have any
suggestions on how to avoid this problem?  The M-1 media is a new bottle
(will not expire until Dec, 2011).  Thank you in advanced for your
suggestions.

 

Best regards,

Joyce Kwan

Elan Pharmaceuticals

South San Francisco, CA

 


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