[Histonet] staining questions

[Joseph Saby]

Betsy-

When staining for proteoglycans, a stain usually associated with Alcian Blue would be PAS.  Stain with Alcian Blue first, then perform the PAS.  A light hematoxylin usually completes the stain.

Joe Saby, BA HT




________________________________
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, February 2, 2010 6:34:04 AM
Subject: [Histonet] staining questions

Good morning!

I will be staining vessels for proteoglycans using Alcian Blue. I was
going to use the 2.5pH  protocol with a Nuclear Fast Red counterstain.
Does anyone have any other suggestion? The investigator does not want
Movats.

Also, I normally use PTAH as my fibrin stain for clots but was thinking
that there may be something better. I found the Fraser-Lendrum but the
protocol stated that Zenkers was the best fixative and that is out of
the question. So again, any suggestions? Thank you in advance.



Betsy Molinari HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812



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