[Histonet] IHC protocol for vimentin in animal tissue

[Jan Shivers]

Lynn,

I don't know anything about the Ventana Benchmark XT requirements, but I 
have never had any problem with Vimentin on a Dako Autostainer and also done 
manually on all sorts of animal tissue - with the exception of mouse.  The 
V9 clone doesn't work on mouse, in my experience.  (It'll work on frogs, 
however; go figure.)

My protocol uses Dako Target Retrieval in a Biocare Decloaking Chamber for 
antigen retrieval, 45 min in primary antibody, 30 min in EnVision+/HRP goat 
anti-mouse IgG, and 10 minutes in AEC.  Very strong staining.

Hope you get the specific answers you need from others.
Jan Shivers
UMN Vet Diag Lab

----- Original Message ----- 
From: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, February 02, 2010 12:50 PM
Subject: [Histonet] IHC protocol for vimentin in animal tissue


Does anyone out there have an IHC protocol, for Vimentin, in animal tissues, 
that has been working for them on the Ventana Benchmark XT? I would 
appreciate any suggestions at this point.

Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
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