[Histonet] Klotz fixative

[Greg Dobbin]

The benefit of Klotz is it preserves tissues/organs while not altering
the color and texture "much". Therefore good for demonstration labs. We
used to store teaching specimens in Klotz in the walk-in cooler (+ 4C)
to aid in the preservation even further. It is not adequate for
long-term storage as far as I am aware. I have no experience with the
affects on histological preparations.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> John Kiernan <jkiernan <@t> uwo.ca> 2/1/2010 2:08 AM >>>
A Google search for KLOTZ FIXATIVE brings up many hits, with
indications that "Klotz solution" is an embalming fluid for gross
anatomy.  That's not the same as as a fixative. 
 
Follow up the links and see if they match what you need.  
Embalming fluids do not necessarily provide good histological fixation.

 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Date: Friday, January 29, 2010 16:00
Subject: [Histonet] Klotz fixative
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>

> 
> Do you know of anyone that sells Klotz fixative?  We use it 
> in a pre vet class but would like to eliminate the 
> choloralhydrate since it is such a hassel due to it's being a 
> controlled substance. Is there a substitute fixative that would 
> retain the properties of Klotz?
> 
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