[Histonet] Bouins and the Trichome
Parker, Helayne
HParker <@t> Skaggs.Net
Wed Dec 29 14:26:22 CST 2010
We have a stain through Sigma (and we change out solutions from Poly Sci too) that we place the slides in a plastic coplin jar w/ Bouins (w/a hole drilled in the top) and microwave for 25 seconds and let set for 5 mins. Then run slides in running water until clear. Tap water is fine around here. Then at that point we continue the stain at room temperature w/ regular times. It is a Masson method and is turns out very nicely- very deep rich reds and blues.
Helayne Parker, HT, (A.S.C.P.)
Skaggs Regional Medical Center
Branson, Missouri
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 29, 2010 12:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 85, Issue 27
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Today's Topics:
1. Topic 5: Tissue Processor Selection (Maglione, Marilou (Muscato))
2. Experienced Histotech and Histology Supervisor Jobs (Alisha Dynan)
3. RE: Chuck Churukian (Tony Henwood)
4. Fwd: Nor Cal Histotech program (Jonathan Krupp)
5. Re: Fwd: Nor Cal Histotech program (Lee & Peggy Wenk)
6. Re: bouin solution (Lee & Peggy Wenk)
7. Florida State Histology License (Kim Tournear)
8. CAP question for pediatric pathology labs (Houston, Ronald)
9. AgNORs staining prtocol & UVC lamp safeguard
(tahseen <@t> brain.net.pk)
10. RE: Florida State Histology License (histotech <@t> imagesbyhopper.com)
11. billing for breast cases (Hutton, Allison)
12. Re: billing for breast cases (DKBoyd <@t> chs.net)
----------------------------------------------------------------------
Message: 1
Date: Tue, 28 Dec 2010 13:52:48 -0500
From: "Maglione, Marilou (Muscato)" <mmaglion <@t> unch.unc.edu>
Subject: [Histonet] Topic 5: Tissue Processor Selection
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <A358777D4CF2554C89666B8C3509759E078CE1 <@t> MSX4.unch.unc.edu>
Content-Type: text/plain; charset=iso-8859-1
Hi Histonetters,
I thought I'd weigh in on the question about tissue processors...I've been a 'lurker' and have enjoyed everyone's helpful posts. Thanks!
We have had VIP's for many years, with dependable service. But through our efforts to make our lab Lean and more efficient, we've added 3 Peloris processors over the past 2 years. The tissue quality is excellent and the processing times are less than half that of our VIP's. We process fatty tissues in 5.5 hours now and most rushes in 45 min. Each Peloris can function as two separate processors, so for a small lab one retort could be used for rushes while a full-length program is running in the other. We also use less reagent because the Peloris tells us when to change the solutions, helping us avoid extreme variations in reagent quality and wasting reagents that are still good. Our pathologists have been very happy with the quality and the TAT and everyone finds it really easy to use.
Marilou Maglione CT (ASCP), HTL
UNC Healthcare
Chapel Hill, NC
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 28, 2010 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 85, Issue 26
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Today's Topics:
1. RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding
procedure manual - Email found in subject (Kim.Donadio <@t> bhcpns.org)
2. Chuck Churukian's Address (Akemi Allison)
3. Use of ammonia water (Sharon.Davis-Devine)
4. What New Tissue Processor Should we get ? (Parker, Helayne)
5. RE: What New Tissue Processor Should we get ? (Nails, Felton)
6. Listing (M.N.)
7. RE: Use of ammonia water (Susan.Walzer <@t> HCAHealthcare.com)
8. RE: Use of ammonia water (Bartlett, Jeanine (CDC/OID/NCEZID))
9. RE: MPB antibody (Settembre, Dana)
10. bouin solution (wassan alkadhumi)
11. AW: [Histonet] bouin solution (Gudrun Lang)
12. Re: Use of ammonia water (Victoria Baker)
----------------------------------------------------------------------
Message: 1
Date: Mon, 27 Dec 2010 14:28:24 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding
procedure manual - Email found in subject
To: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF15A439EE.16C8EA1C-ON86257806.00702344-86257806.007076F0 <@t> bhcpns.org>
Content-Type: text/plain; charset="US-ASCII"
Do others document separately the original creation, training, competency,
implementation of procedures/processes and then continual yearly training,
competency and review of those processes? Are you using a Quality
Management System? How about Electronic system to manage?
Yes, to all of those. We document original training and then all
competencies are part of their yearly eval. We also have the QMS
electronic policy/procedure Data base that sends each individual reminders
when they need to read a update or sign off on the yearly requirement that
they have read and understand the material.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/23/2010 10:49 AM
To
<rcazares <@t> schosp.org>, histonet <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual -
Email found in subject
Thanks, I am aware of Peggy's form. We have similar hard copy forms now,
but we are moving to an electronic system. It will be much more manageable
for 125 employees.
When you state in your form the employee is competent, do you have
documentation to support that there was a competency evaluation? We only
state the employee has read and understands. We have separate
documentation for training and competency.
I love this type of discussion string. There are so many different
processes out in Histoland.
Do others document separately the original creation, training, competency,
implementation of procedures/processes and then continual yearly training,
competency and review of those processes? Are you using a Quality
Management System? How about Electronic system to manage?
William DeSalvo, B.S., HTL(ASCP)
> From: RCazares <@t> schosp.org
> To: wdesalvo.cac <@t> hotmail.com
> Date: Thu, 23 Dec 2010 10:17:48 -0600
> Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure
manual - Email found in subject
>
> Hello histonetters,
>
> When I implement a new procedure in my lab, besides the routine yearly
sign-off sheet that accompanies the procedure, there an additional sheet
that states
>
> " The following employees have reviewed this procedure and by signing
below are acknowledging that they understand and are competent in
performing the procedure:"
>
> Below that statement is a box with signature and date lines for all the
employees using this procedure to sign. This makes each employee
responsible for reading and understanding the procedure. This I got from
one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to
anyone who'd like one, just contact me directly.
>
>
> Ruth Cazares, HT (ASCP)
> Histology Supervisor
> Department of Pathology
> Swedish Covenant Hospital
> Chicago, IL
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
> Sent: Thursday, December 23, 2010 7:27 AM
> To: bsullivan <@t> shorememorial.org; Victor Tobias
> Cc: histonet; histonet-bounces <@t> lists.utsouthwestern.edu
> Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure
manual - Email found in subject
>
>
> Beatrice,
> How do you show proof to CAP that your employees that use the procedures
and perform the tasks described in the procedures have reviewed and
understand the procedure when there is a new one during your cycle year
and before it is implemented?
>
> CAP has changed the way they inspect and they are now looking for how
whether the employees performing a task described in the procedure
understands the procedure and is performing exactly as the procedure
describes. They are not as concerned that the Supervisor and management
knows about the procedures, they want to see how informed, trained and
competent the bench employee is.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
> > To: victor <@t> pathology.washington.edu
> > From: BSullivan <@t> shorememorial.org
> > Date: Thu, 23 Dec 2010 07:53:22 -0500
> > Subject: Re: [Histonet] CAP Question regarding procedure manual
> > CC: Histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
> >
> > Victor,
> > To my knowledge all you need is proof that the staff reviewed the
manuals.
> > We accomplish this by a sign off sheet in the front of each manual we
use.
> > The Supervisor, or designee, needs to review and sign off on each
procedure
> > in each manual.
> >
> > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
> > AP Supervisor
> > Shore Memorial Hospital
> > 609-653-3590
> >
> >
> > Speak only well of people and you need never whisper
> >
> >
> >
> > Victor Tobias
> > <victor <@t> pathology
> > .washington.edu> To
> > Sent by: Histonet
> > histonet-bounces@ <Histonet <@t> lists.utsouthwestern.edu>
> > lists.utsouthwest cc
> > ern.edu
> > Subject
> > [Histonet] CAP Question regarding
> > 12/22/2010 06:16 procedure manual
> > PM
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > Is there a requirement to have a signature of every staff member on a
> > procedure if they perform that procedure in a manual? Wouldn't one
> > signature on a cover page suffice that you have read and understand
what
> > is in the manual?
> >
> > Victor
> >
> > --
> > Victor Tobias
> > Clinical Applications Analyst
> > University of Washington Medical Center
> > Dept of Pathology Room BB220
> > 1959 NE Pacific
> > Seattle, WA 98195
> > victor <@t> pathology.washington.edu
> > 206-598-2792
> > 206-598-7659 Fax
> > =================================================
> > Privileged, confidential or patient identifiable information may be
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
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Message: 2
Date: Mon, 27 Dec 2010 12:57:31 -0800 (PST)
From: Akemi Allison <akemiat3377 <@t> yahoo.com>
Subject: [Histonet] Chuck Churukian's Address
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <563441.88990.qm <@t> web113805.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All,
I just spoke with Chuck a few minutes ago and his spirits are up.? He?is working
with a physical therapist this afternoon.? He gave me permission to post his
address, and would love to hear from those of you who would like to send him
cards.? I think it would really cheer him up.?
His address is:
34 Gailhaven Court
Rochester, NY 14618
?Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
------------------------------
Message: 3
Date: Mon, 27 Dec 2010 14:58:20 -0600
From: Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Use of ammonia water
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE4513DAAA85804D9A9FBAC9C1EE7A668EDBEE75 <@t> EXCHANGECCABE.carle.com>
Content-Type: text/plain; charset="us-ascii"
Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com
------------------------------
Message: 4
Date: Mon, 27 Dec 2010 15:15:44 -0600
From: "Parker, Helayne" <HParker <@t> Skaggs.Net>
Subject: [Histonet] What New Tissue Processor Should we get ?
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<930EB2E8DF68C544873EDD2A3D5F506004700E6898 <@t> email1.skaggs.net>
Content-Type: text/plain; charset="us-ascii"
Hi All,
If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly.
Any input is much appreciate on these or other products - private emails great !
Thanks,
Helayne Parker
hparker <@t> skaggs.net
CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
------------------------------
Message: 5
Date: Mon, 27 Dec 2010 15:19:37 -0600
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: [Histonet] RE: What New Tissue Processor Should we get ?
To: "'Parker, Helayne'" <HParker <@t> Skaggs.Net>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1FE5960057C084CA389CE977790629090652BDB <@t> TCDMSG01.ad.TexasChildrensHospital.org>
Content-Type: text/plain; charset=us-ascii
As have you, I have also had great service from my VIP's and plan on purchasing the VIP 6.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Parker, Helayne
Sent: Monday, December 27, 2010 3:16 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] What New Tissue Processor Should we get ?
Hi All,
If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly.
Any input is much appreciate on these or other products - private emails great !
Thanks,
Helayne Parker
hparker <@t> skaggs.net
CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
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Message: 6
Date: Mon, 27 Dec 2010 22:04:46 -0800
From: "M.N." <mnglegal <@t> gmail.com>
Subject: [Histonet] Listing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTinK1Sosw5ScH7KNHL7ajFxMVep__S5Dv1h0UiZD <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi - I am looking for someone in the LA area (ideally someone who works in a
lab or hospital setting) to teach me how to process biopsies from start to
finish, and to cut paraffin-embedded sections. I would compensate you for
your time, of course. Please contact me with your availability - I'd like
to get started soon. Thanks!
------------------------------
Message: 7
Date: Tue, 28 Dec 2010 01:57:56 -0600
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: [Histonet] RE: Use of ammonia water
To: <Sharon.Davis-Devine <@t> carle.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BF03F5404EBDE409AF9232DA74B9DED2B3D09D7CA <@t> FWDCWPMSGCMS09.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water
Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 28 Dec 2010 05:51:55 -0500
From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Use of ammonia water
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<E129883C1CDAD2438D2680E08E0C2A0DAFC306 <@t> LTA3VS022.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
I use it on particularly difficult blocks and have seen no ill effects.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water
Happy Holidays Histonetters, I have a question for you. Do any of you
use ammonia water to soften the blocks before cutting? If so, does this
have any effect on IHC staining? Any help will be greatly appreciated.
Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 28 Dec 2010 09:40:19 -0500
From: "Settembre, Dana" <settembr <@t> umdnj.edu>
Subject: [Histonet] RE: MPB antibody
To: "'Taylor, Jean'" <jtaylor <@t> meriter.com>, "'ihcrg <@t> googlegroups.com'"
<ihcrg <@t> googlegroups.com>, "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B64B947688FB794A8C191D19F22927C875BE700A <@t> UMDEXMBX02.core.umdnj.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Jean,
I use Leica's MBP which the get from NovoCastra cat. NCL-MBP
I use it on formalin fixed paraffin embedded human tissue on my Dako.
I use Dako's Target Ret. Soln in a steamer for 40 minutes
Incubate 15 min with it at the dilution of 1:25.
Then I use Dako LSAB2 kit.
It works fine.
Good luck,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Thursday, December 23, 2010 2:14 PM
To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] MPB antibody
Hi Everyone,
Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation.
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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------------------------------
Message: 10
Date: Tue, 28 Dec 2010 06:49:54 -0800 (PST)
From: wassan alkadhumi <w_alkadhumi <@t> yahoo.com>
Subject: [Histonet] bouin solution
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <604286.3701.qm <@t> web45206.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Histonet
I have question concerning?bouin solution, we use it in masson trichrome before
starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution
for 18 hr in room temp which is not controlled.?Then?we wash the slides?using
running water first and?then distilled water, but?according to what
i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)%
alcohol?to prevent deterioration of staining in time. now i should mention?that
in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only
with water.
what should i do? In the book its mentioned that we have to wash with alcohol if
the tissue is not processed yet, is that why the alcohol step is?missing in
masson tri procedure?and why will the stain deteriorate if some of the bouin
solution stayed in the tissue?
The?main fixative that is used in our lab for all grooses and biopsy is 10%
formalin (except bone marrow biopsy). bouin solution is used after processing of
the tissue and deparaffinization of the sections is finished.
Thanks
Wassan
Histotechnician
Shorh hospital
North of Iraq
------------------------------
Message: 11
Date: Tue, 28 Dec 2010 17:42:52 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] bouin solution
To: "'wassan alkadhumi'" <w_alkadhumi <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2CFC6E180EFB469AA061237DC32D690B <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
We incubate the trichrome-slides after deparaffination for 2 hours in 56?C,
then rinse in warm tapwater until the slides are colourless. Then another
rinse in distilled water, because I don't want carry over from tapwater into
the weigert's iron hematoxylin. ...
If you let bouins in the tissue, it acts like a stain. Think of vanGieson
staining solution (also called picrofuchsin).
It sounds like the processing after bouin-fixation and the trichrom staining
are mixed up. Or the protocols of trichrom-staining of NBF-fixed and
bouin-fixed tissue are mixed up.
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von wassan
alkadhumi
Gesendet: Dienstag, 28. Dezember 2010 15:50
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] bouin solution
Dear Histonet
I have question concerning?bouin solution, we use it in masson trichrome
before
starting the stain.?we incubate the slides trimmed at 3 micron in bouin
solution
for 18 hr in room temp which is not controlled.?Then?we wash the
slides?using
running water first and?then distilled water, but?according to what
i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)%
alcohol?to prevent deterioration of staining in time. now i should
mention?that
in the?our ?masson trichrome?procedure?there is no washing with alcohol?but
only
with water.
what should i do? In the book its mentioned that we have to wash with
alcohol if
the tissue is not processed yet, is that why the alcohol step is?missing in
masson tri procedure?and why will the stain deteriorate if some of the bouin
solution stayed in the tissue?
The?main fixative that is used in our lab for all grooses and biopsy is 10%
formalin (except bone marrow biopsy). bouin solution is used after
processing of
the tissue and deparaffinization of the sections is finished.
Thanks
Wassan
Histotechnician
Shorh hospital
North of Iraq
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Tue, 28 Dec 2010 12:47:13 -0500
From: Victoria Baker <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] Use of ammonia water
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AANLkTimche31m+13x0BUSNN+j_7UkHUMZGCHgLk1DSGM <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Sharon,
I use a 1.5% solution for bone marrow cases and bloody tissue. It does also
work well for GI. I've never had an issue with it affecting any of my IHC.
An alternative though (when I can't get my hands on ammonium hydroxide) is
to put these blocks in warm water (I'm careful about the waterbath because
of X-contamination issues) for about 30-45 seconds and this works just as
well. I stopped using any surface decal solution or Nair as I did see some
differences with the expression of certain markers - I did not see any false
negs, but the staining itself was not as intense it looked like.
Vikki
On Mon, Dec 27, 2010 at 3:58 PM, Sharon.Davis-Devine <
Sharon.Davis-Devine <@t> carle.com> wrote:
> Happy Holidays Histonetters, I have a question for you. Do any of you use
> ammonia water to soften the blocks before cutting? If so, does this have any
> effect on IHC staining? Any help will be greatly appreciated. Thanks.
>
> Sharon Davis-Devine, CT (ASCP)
> Cytology-Histology Supervisor
> Carle Foundation Hospital
> Laboratory and Pathology Services
> 611 West Park Street
> Urbana, Illinois 61801
> 217-383-3572
> sharon.davis-devine <@t> carle.com
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
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End of Histonet Digest, Vol 85, Issue 26
****************************************
------------------------------
Message: 2
Date: 28 Dec 2010 16:10:21 -0500
From: Alisha Dynan <alisha <@t> ka-recruiting.com>
Subject: [Histonet] Experienced Histotech and Histology Supervisor
Jobs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <322850652.1293570621270.JavaMail.cfservice <@t> SL4APP4>
Content-Type: text/plain; charset="utf-8"
Dear Histonet Members,
I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses.
I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions:
* Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus
* Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must
They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.
If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.
To view some additional opportunities please visit our website at www.ka-recruiting.com.
Sincerely,
Alisha (Taylor) Dynan, Founder
K.A. Recruiting, Inc.
Your Partner in Healthcare Recruiting
10 Post Office Square 8th Floor SOUTH
Boston, MA 02109
P: (617) 692-2949
F: (617) 507-8009
alisha <@t> ka-recruiting.com
www.ka-recruiting.com
------------------------------
Message: 3
Date: Tue, 28 Dec 2010 21:51:53 +0000
From: Tony Henwood <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Chuck Churukian
To: "'Akemi Allison'" <akemiat3377 <@t> yahoo.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A71570358AB <@t> xmdb02.nch.kids>
Content-Type: text/plain; charset="us-ascii"
What disturbing news.
Hopefully Chuck can fight on and be back to full strength by April.
All our prayers and support are for Chuck and his family.
A father figure in Histochemistry.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Monday, 27 December 2010 12:12 PM
To: Histonet
Subject: [Histonet] Chuck Churukian
I just heard from Irene Churukian today. Please put Chuck in your thoughts and prayers. Irene said this:
CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether - three weeks apart. The first cycle was difficult... he was very weak.... in bed most of the time.
He is now improving ... thankfully. He has never been sick and confined to bed/home so he gets cabin fever easily. People have been so kind to visit him. So our Christmas this year was very different from years past. We are hoping and praying that by April, he will be in remission after all the treatments are completed.
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
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------------------------------
Message: 4
Date: Tue, 28 Dec 2010 15:22:54 -0800 (PST)
From: Jonathan Krupp <jkrupp <@t> deltacollege.edu>
Subject: [Histonet] Fwd: Nor Cal Histotech program
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<531815146.272564.1293578574576.JavaMail.root <@t> mercury.deltacollege.edu>
Content-Type: text/plain; charset=utf-8
Hi
I am new here. I am part of an EM tech training program at Delta College in Stockton, CA.
We train students to do EM, fixing, sectioning, staining, and instrument operation.
Some of my students have been asking about LM histotech sort of things. They want to know more about histo techniques in general and maybe broadening their job prospects beyond EM.
I am exploring the possibilities of adding more LM histotech things to the program and seek advice. Is there room for another program? Is seeking accreditation worth while? What would I need to get set up? Are there jobs available for graduates?
Just getting started, your advice will be invaluable.
Jon
Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA 95207
209-954-5284
jkrupp <@t> deltacollege.edu
------------------------------
Message: 5
Date: Tue, 28 Dec 2010 19:48:02 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Fwd: Nor Cal Histotech program
To: "Jonathan Krupp" <jkrupp <@t> deltacollege.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <587BDAFD5B6A4379A22840FB4B8A03A0 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="UTF-8";
reply-type=original
There is SUCH a need for histotech programs. Currently, there are only 39
histologic technician (HT) (most community college based) and 4
histotechnologist (HTL) (need 4 year degree). And there are shortages
everywhere.
There are 2 HT programs in California. One in Berkley, one in Walnut.
The accrediting agency is National Accrediting Agency for Clinical
Laboratory Sciences (NAACLS).
www.naacls.org
For information on how to contact the two California programs, once on the
NAACLS website,
- on the left, click on Find a Program
- Under Program Type, click on HT & hit Search. Programs are listed
alphabetically by state.
- If you want to talk with HTL program directors, follow above directions,
except click on HTL.
Any program director would be willing to give you more information.
If you look under "accreditation" on the left, there is HT and HTL. Click on
one, and you can find the accreditation standards you would have to follow.
There are a few standards that are different between HT and HTL, so you need
to look at both.
The advantage of being an accredited HT or HTL program is that the students
are eligible to take the national certification exam through American
Society for Clinical Pathology (ASCP), immediately upon successful
completion of the NAACLS accredited HT or HTL program.
However, since you are just starting out, and look like you need lots of
information, I'm going to suggest going to the National Society for
Histotechnology (NSH) website. More information about what histotechs are,
about meetings, state society presidents, jobs board, membership with
journal specific for histology/tissue, etc.
There is also a 3 hour continuing education you can purchase, titled "How to
Start an Accredited HT or HTL Program in either a College or a Hospital". I
presented it at the NSH Symposium in 2008, and it was recorded with the
PowerPoint, so you can listen to me and look at the PowerPoint. It has lots
of advantages and disadvantages of starting different types of programs,
contacts, proof that we need more programs, etc. Granted, the information is
3 years old, and there are a lot more HT and HTL program than there were 3
years ago, but most of the other information is the same. Just a disclaimer,
I don't make any money from this. I had presented it every year from
1999-2008, and NSH thought it might work as an on-line module, for people
like yourself who need the information at times other than in the fall when
the NSH symposium is.
Go to www.nsh.org
- Across the top, click on Professional Development
- About the 5th one down, click on On-Line Learning Center
- Click on Meeting Content
- Scroll down and click on NSH 2008 Annual Symposium/Convention in
Pittsburgh, PA
- Click on #27 = How to Start an Accredited HT or HTL program
Once you get some information under your belt, I'd be more than happy to
answer questions specific for your situation. Just look on the NAACLS web
page, under HT or HTL (we have both programs), and you'll find me in
Michigan. :-)
Peggy A. Wenk, HTL(ASCP)SLS
Schools of Histotechnology
William Beaumont Hospital
Royal Oak, MI 48073
--------------------------------------------------
From: "Jonathan Krupp" <jkrupp <@t> deltacollege.edu>
Sent: Tuesday, December 28, 2010 6:22 PM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Fwd: Nor Cal Histotech program
>
>
>
> Hi
>
> I am new here. I am part of an EM tech training program at Delta College
> in Stockton, CA.
>
> We train students to do EM, fixing, sectioning, staining, and instrument
> operation.
>
> Some of my students have been asking about LM histotech sort of things.
> They want to know more about histo techniques in general and maybe
> broadening their job prospects beyond EM.
>
> I am exploring the possibilities of adding more LM histotech things to the
> program and seek advice. Is there room for another program? Is seeking
> accreditation worth while? What would I need to get set up? Are there jobs
> available for graduates?
>
> Just getting started, your advice will be invaluable.
>
> Jon
>
> Jonathan Krupp
> Delta College
> 5151Pacific Ave.
> Stockton, CA 95207
> 209-954-5284
> jkrupp <@t> deltacollege.edu
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Tue, 28 Dec 2010 20:18:03 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] bouin solution
To: "wassan alkadhumi" <w_alkadhumi <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <F9B870501B5C4E11A66E1B53B7109195 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
If the tissue is in Bouins, the excess Bouins needs to be washed out of the
tissue immediately after fixation.
Water could be used to rinse out the excess picric acid, but I think some
people are worried that there could be some cell swelling if the tissue is
left in the water too long (particularly if the tissue is underfixed/not
stablized enough).
If I remember correctly, too high of a percent of alcohol (95%, for
example), will cause the picric acid salts to precipitate in the tissue, and
it would be like trying to microtome tissue with sand in it.
So 50-70% alcohol is a good compromise. Have enough water to remove the
excess picric acid salts, but not too much water to cause tissue swelling,
and not too much percent of alcohol to cause picric acid salts to
precipitate in the tissue.
For your slides already microtomed and on slides - these have already been
fixed and processed. If the tissue was fixed in a picric acid fixative like
Bouins, there is no need for the step of putting the slide in Bouins. If the
tissue was fixed in something else (10% neutral buffered formalin, for
example), then the slide/tissue needs to be placed into Bouins in order for
the trichrome stains to work correctly. This is called either post-fixing or
post-mordanting. There are several theories as to why the Bouins is needed
for the trichromes (change tissue charges, change tissue density), but the
end result is that Bouins fixed or Bouins post-mordanted tissue have muscles
that are red and collagen that are blue or green (depending upon which dye
you use). If the NBF fixed tissue is not post-mordanted, the red is a
red/blue or red/green, and the collagen has strands of red in it. In other
words, the colors are not as true or as crisp.
Now, the slides that were post-mordanted in Bouins have tissue that is
yellow. We need to get the yellow out. Not so much because of the picrate
salts that precipitate, but more because the yellow color would interfere
with the reds and the blues or greens colors that you are trying to make.
The tissue is only 3-5 um thick, so it won't take as long to remove the
color. And the tissue has been well fixed, dehydrated and cleared during
tissue processing. So there are lots of cross-links to stabilize the tissue.
So rinsing in water is only going to take a few minutes, and the tissue is
stabilized, so there shouldn't be any cell size changes. You could rinse in
50-70% alcohol, but that's only 50-30% water, so it is going to take a lot
longer to get rid of the yellow color than rinsing in water.
Rinsing in tap water is fine for most labs, as long as the quality of tap
water is good (not too acidic or basic, not too much iron or sulfur, no
microorganisms). I like to rinse with d. water before any stain, just in
case there is any contaminating metal that might interfere with the stains
(we have a lot of iron in our part of the state).
By the way, putting the slides in Bouins in a coplin jar with a lid screwed
on, then putting in a 60 degree C. oven or water bath for 45-60 minutes
works great. This would reduce your turn around time by 17 hours! Two words
of caution. 1) Don't breath in the hot Bouins fumes (picric acid, acetic
acid, formaldehyde). Open up the coplin jar in a hood. 2) Don't stick the
hot coplin jar into cold running water. Coplin jar will break/crack. We
usually take the slides out of the hot Bouins, and put the slides into a new
coplin jar with water, then put this under the running water. The lid is put
back on the Bouins coplin jar, and allowed to cool. Some places reuse the
Bouins for a number of times.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
--------------------------------------------------
From: "wassan alkadhumi" <w_alkadhumi <@t> yahoo.com>
Sent: Tuesday, December 28, 2010 9:49 AM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] bouin solution
> Dear Histonet
> I have question concerning bouin solution, we use it in masson trichrome
> before
> starting the stain. we incubate the slides trimmed at 3 micron in bouin
> solution
> for 18 hr in room temp which is not controlled. Then we wash the slides
> using
> running water first and then distilled water, but according to what
> i red, when using bouin solution,the tissue should be washed with (50-70)%
> alcohol to prevent deterioration of staining in time. now i should mention
> that
> in the our masson trichrome procedure there is no washing with alcohol
> but only
> with water.
>
> what should i do? In the book its mentioned that we have to wash with
> alcohol if
> the tissue is not processed yet, is that why the alcohol step is missing
> in
> masson tri procedure?and why will the stain deteriorate if some of the
> bouin
> solution stayed in the tissue?
> The main fixative that is used in our lab for all grooses and biopsy is
> 10%
> formalin (except bone marrow biopsy). bouin solution is used after
> processing of
> the tissue and deparaffinization of the sections is finished.
> Thanks
>
> Wassan
> Histotechnician
> Shorh hospital
> North of Iraq
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 29 Dec 2010 05:10:01 -0800 (PST)
From: Kim Tournear <kim.tournear <@t> yahoo.com>
Subject: [Histonet] Florida State Histology License
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <313964.32140.qm <@t> web120203.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Can any one tell me how to go about getting a Florida State Histology License? And I understand that there is a test to take for the Supervisor's license as well....I'll take info for that too,,,,thanks in advance!!
~Kim~? ?
OU ROCKS!!!!
~Don't be afraid your life will end,
be afraid it will never begin~
------------------------------
Message: 8
Date: Wed, 29 Dec 2010 09:12:50 -0500
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] CAP question for pediatric pathology labs
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E02E1309B208F94C83B968E45781001A234C53BBC6 <@t> NCHEXMBX01.columbuschildrens.net>
Content-Type: text/plain; charset="us-ascii"
CYP.00190
Phase I
N/A YES NO
For laboratories that perform non-gynecologic cytopathology, does the laboratory participate in a peer educational program in NON-GYNECOLOGIC cytopathology (e.g., CAP Interlaboratory Comparison Program in Non-Gynecologic Cytopathology NGC)?
How are labs responding to this request for a peer-educational program in non-gyn cytopath, as there is no such program for pediatric cytopathology, and, in the opinion of our pathologists, the CAP program is useless as far as it pertains to pediatrics?
We have had varying responses from inspectors which we have adopted and then the next inspector says this isn't good enough and you should be doing something else. There has been little to no direction from CAP.
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>
"One person with passion is better than forty people merely interested."
~ E.M. Forster
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------------------------------
Message: 9
Date: Wed, 29 Dec 2010 19:23:28 +0500 (PKT)
From: tahseen <@t> brain.net.pk
Subject: [Histonet] AgNORs staining prtocol & UVC lamp safeguard
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <25574.203.135.35.66.1293632608.squirrel <@t> brain.net.pk>
Content-Type: text/plain;charset=iso-8859-1
Dear All,
1 Would you like to share your AgNORs staining prtocol with me?
2 We are using Leica CM1850 US Cryostat, we are going to use UVC lamp for
disinfection, so what kind of safeguard are recommended other then sliding
window has been properly closed.
Thanks advance
Muhammad Tahseen
Senior Supervisore
Histopathology
SKMCH & RC
Lahore Pakistan.
------------------------------
Message: 10
Date: Wed, 29 Dec 2010 10:42:56 -0500
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] Florida State Histology License
To: "'Kim Tournear'" <kim.tournear <@t> yahoo.com>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002a01cba76f$17e1bef0$47a53cd0$@imagesbyhopper.com>
Content-Type: text/plain; charset="us-ascii"
Kim,
You will have to go to the Florida Department of Health website to get the
application. For a supervisor license, you will need to meet specific
requirements and then take an additional 24 hours of CEUs and pay the
license fee. Please click on the following link for more information.
http://www.doh.state.fl.us/mqa/ClinLab/index.html
There are a number of links on that page, you might wish to begin with the
Applicant Information link.
Good Luck!
Michelle
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Tournear
Sent: Wednesday, December 29, 2010 8:10 AM
To: Histonet
Subject: [Histonet] Florida State Histology License
Can any one tell me how to go about getting a Florida State Histology
License? And I understand that there is a test to take for the Supervisor's
license as well....I'll take info for that too,,,,thanks in advance!!
~Kim~
OU ROCKS!!!!
~Don't be afraid your life will end,
be afraid it will never begin~
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 11
Date: Wed, 29 Dec 2010 12:29:23 -0500
From: "Hutton, Allison" <AHutton <@t> dh.org>
Subject: [Histonet] billing for breast cases
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<38A56C4F4630D348A50B3720409270870E0FE294 <@t> dhmail.dhorg.org>
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A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307?
Thank you in advance,
Allison
------------------------------
Message: 12
Date: Wed, 29 Dec 2010 12:54:16 -0500
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] billing for breast cases
To: "Hutton, Allison" <AHutton <@t> dh.org>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFC50AA239.892D76F3-ON85257808.006229AD-85257808.00625A5C <@t> chs.net>
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My understanding is: Inked margins (on any specimen type) are 88307.
Doesn't specify who inks the margin.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Hutton, Allison" <AHutton <@t> dh.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/29/2010 12:35 PM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] billing for breast cases
A debate has arisen and I am looking to histonet for a more definitive
answer. We have a breast surgeon who, after he removes the lumpectomy
specimen, always goes back and removes more tissue around the margins of
the lumpectomy. Our question is how should these additional margins be
charged. Currently we charge an 88305 for each of the additional margins
(there are no sutures indicating any orientation, however, one can
determine the old and new margins). Is 88305 the correct charge in this
situation or should they be higher at an 88307?
Thank you in advance,
Allison
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End of Histonet Digest, Vol 85, Issue 27
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