[Histonet] RE: MPB antibody

Settembre, Dana settembr <@t> umdnj.edu
Tue Dec 28 08:40:19 CST 2010

Hi Jean,
I use Leica's MBP which the get from NovoCastra cat. NCL-MBP
I use it on formalin fixed paraffin embedded human tissue on my Dako.
I use Dako's Target Ret. Soln in a steamer for 40 minutes
Incubate 15 min with it at the dilution of 1:25.
Then I use Dako LSAB2 kit.
It works fine.
Good luck,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Thursday, December 23, 2010 2:14 PM
To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] MPB antibody

Hi Everyone,

Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation.


Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

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