[Histonet] EM cacodylate
Timothy.Morken <@t> ucsfmedctr.org
Mon Dec 20 10:03:08 CST 2010
Peggy, Carson's modification of Millonig's buffer with formalin is great for kidney and any other general EM use for general pathology labs. I used it for many, many years when I worked in EM and it worked just as well as the specialized EM glutaraldehyde fixatives / buffers.
Just look up "Carson millonig" and you'll find several commercial sources. Or, of course, look at Carson's book for the formula.
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
Sent: Saturday, December 18, 2010 4:26 AM
Subject: [Histonet] EM cacodylate
Need some help from Electron Microscopy people.
What is the best substitute for cacodylate buffer (arsenic) when using on kidney and nerve biopsies? Least amount of protein clumping, little to now salt precipitate, good EM resolution?
Thanks in advance.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
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