[Histonet] Decalcification

Sherwood, Margaret MSHERWOOD <@t> PARTNERS.ORG
Wed Dec 8 12:45:04 CST 2010

Could you explain in more detail the process, Liz?



-----Original Message-----
From: Liz Chlipala [mailto:liz <@t> premierlab.com] 
Sent: Wednesday, December 08, 2010 1:47 PM
To: Sherwood, Margaret ; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Decalcification

Try surface decaling the samples in the block.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret 
Sent: Wednesday, December 08, 2010 11:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Decalcification

We have small arteries that were FFPE and sectioned that should have
decacified before processing.  (The investigator did not indicate as
such when
submitting them).  Cutting was extremely difficult and the sections, as
you can
imagine, had terrible knife marks and chatter.  Of course, they are
(for a paper)!  

Is there any way tissue, that has been embedded, can be decalcified at
point?  Or some other way to treat the block so that we can get better

Thanks for all your help.


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 

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